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Microarray Tools - 384-Well Microplates


Arrayit's ArrayIt® Brand Microarray Microplate 384 (MMP384) sample plates set a new standard for microarray research.  Rigid polypropylene construction and well design make this product ideal for use with Arrayit’s patented 946, Professional, Stealth and ChipMaker Micro Spotting Technology, and high-end microarray robots equipped with plate and lid handling systems. The unique well geometry and surface properties expedite the use of small sample volumes.


Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Plate Specifications
  • Short Protocol
  • Complete Protocol
  • Troubleshooting Tips
  • Ordering Information
  • Storage Conditions
  • Warranty

Introduction
Congratulations on taking a big step towards improving the affordability, quality and speed of your genomics, biomedical, pharmaceutical and agricultural research.  This booklet contains all the information required to take full advantage of Arrayit's ArrayIt Brand Microarray Microplate 384 (MMP384).

Quality Control
Arrayit takes every measure to assure the quality of each microplate and microplate cover, and guarantees their use in microarray applications.

Product Description
Arrayit's ArrayIt Brand Microarray Microplate 384 provides the highest quality microplate for use in microarray manufacturing and storage at an affordable price. No other microplate on the market can compete with our quality, performance, and affordability.

Users will appreciate the following features:

  • Superior low volume liquid handling
  • Excellent compatibility with the ArrayIt® Microarray Spotting Devices
  • Polypropylene high contact angle produces sample “beading”
  • Polypropylene has low DNA, protein, cell extract, and small molecule binding
  • Deep well depth and narrow well diameter slow sample evaporation
  • Sample volumes of =3.0 µl can be used with our printing pins
  • Heavy-duty rigid construction compatible with automated plate handling systems
  • Ultra-flat design allows true seating on robot platens
  • Highly accurate well-to-well spacing enables easy calibration
  • Free of the electrostatic charge and sample binding problems associated with polystyrene plates
  • Compatible with the VacuSeal™ plate storage system

Plate Specifications

  • Meets all SBS microplate standards
  • Plate size (L, W, H):  12.7 x 8.5 x 1.4 cm
  • Plate weight (without lid):  70 g
  • Well depth:  11.5 mm
  • Well circumference:  3.2 mm
  • Well volume (max): 60 µl
  • Recommended working volume per well: 3-15 µl
  • Flatness (across entire plate): ±500 µm
  • Well markings (left side from top to bottom):  A-P
  • Well markings (top from left to right):  1-24

Figure 1. Schematic diagram depicting how the superior well geometry and polypropylene surface properties enable low volume liquid handling and sample loading. An empty well (left) is loaded with 3 µl of sample (center), allowing easy loading of sample into a Micro Spotting Pin (right).  Recommended working volumes in the Microarray Microplate 384 are 3-15 µl.
Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail:
Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. (under the subject heading please type, "ArrayIt technical assistance"). By telephone:  (408) 744-1331, Monday–Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!


Figure 2. Contact angle measurement. A 1.0-µl droplet of H2O placed on a Microarray Microplate 384 (MMP384) produces a contact angle of 88° (red line).  The high contact angle of polypropylene causes microarray samples to “bead up” in the bottom of the source plate, expediting low volume liquid handling and pin loading.

Figure 3.  Plate wells top and bottom views.  Shown are photographs of the Microarray Microplate 384 (MMP384) viewed from the top (left) and bottom (right). The round 3.2 mm wells and conical well bottoms are clearly visible.


Short Protocol

  1. Set up plates for printing.
  2. Fill plates with samples.
  3. Spin samples to bottom of wells.
  4. Print samples into microarrays.
  5. Seal sample plates for storage.

Complete Protocol

  1. Set up plates for printing.  Remove Microarray Microplates (MMP384) from plastic packages.  Always handle plates with synthetic rubber cleanroom gloves to prevent the transfer of particulates, powder or contaminating latex proteins onto the plates.  Minimizing plate and sample contamination at all points during microarray manufacture will improve microarray quality. Microarray Microplate Covers (MMC384) can also be used to prevent sample contamination and dehydration.
  2. Fill plates with samples.  Prepare purified samples for printing such as PCR products, oligonucleotides, antibodies, proteins, cell extracts, small molecules, or any other sample of interest.  PCR samples should be purified using either the 96- or 384-well PCR Purification Kits.  Purified DNA and protein samples should be re-suspend in an appropriate printing buffer such as Micro Spotting Solution Plus or Protein Printing Buffer, and loaded at 3–15 µl per well into the source plate.  For best results always use ArrayIt Brand Printing Buffers.  Samples should be free of particulates, salts, and other impurities that can clog printing pins and inhibit coupling chemistry. The recommended sample volume is 3-15 µl per well and the total working volume of the plate is 60 µl per well. Liquid handling robots can be used to transfer printing buffer and other reagents (see Fig. 5).
  3. Spin samples to bottom of wells. After 3-15 µl samples are loaded into each well, spin the plates by centrifugation for 1-5 min (500 x g) to ensure that the samples locate to the bottom of each well. Microarray Microplate Covers (MMC384) can be used to prevent dehydration during the centrifugation step.  Plates can be spun in a Savant Speed Vac equipped with a plate rotor, or an equivalent centrifuge. The round geometry and conical bottom of the MMP384 allows the sample to seat uniformly on the well bottom, without adhering to the side of the plate as is observed with square well plates.  Proper sample seating improves improving pin loading and microarray printing. Do not attempt to print microarrays without making certain that the samples are located correctly on the bottom of each sample well.
  4. Print samples into microarrays.  Once samples are mixed, loaded and centrifuged into the bottom of each well, place the plate on the microarrayer platen for printing. Make certain to position the plates accurately on the microarray deck (see Fig. 5).  Automation technology can be used to change plates and remove plate covers during printing. For best results with printed microarrays, use ArrayIt®  brand SuperMicroarray Substrates.  For the ultimate substrate performance, users might also consider Mirror Substrates for enhanced signals and reduced background.
  5. Seal sample plates for storage.  After microarray printing, plates can be sealed and stored at room temperature, 4°C or –20°C for future use. The VacuSeal™ provides an excellent means of vacuum sealing microplates to protect samples against evaporation and environmental damage.

Figure 4.  Plate and plate cover. Shown are a Microarray Microplate 384 (MMP384) and Microarray Microplate Cover 384 (MMC384).  The clear plastic lid prevents sample dehydration during printing and facilitates plate and sample storage.

Figure 5.  Liquid handling. Shown is the Microarray Microplate 384 (MMP384) seated on a liquid handling robot. Printing buffers and other reagents can be transferred to each sample well in an automated manner.

Figure 6.  Microarray printing. The 384 well plate and samples are shown on a microarray platen into which 4 Micro Spotting Pins are entering 4 wells to pick up samples for microarray printing.


Troubleshooting Tips

1. Micro Spotting Pins fail to load samples

  • Samples not seated on the bottom of the plate wells
  • No sample in the wells
  • Pin clogged with particulate or salts (check wash/dry station)

2. Printed spots merge

  • Sample volumes too large (use 3-15 µl per well max)
  • Printing buffer too dilute (use 1X concentration)
  • Spot spacing too close

Recommended Products

ArrayIt® Brand VacuSeal™ plate storage system and foil seals.

Storage Conditions
Arrayit's Microarray Microplate 384 and Microarray Microplate Cover 384 are stable indefinitely at room temperature (25°C). Plates and covers should be stored in the sealed plastic shipping envelopes.  Avoid exposure to elevated temperature, humidity, and dust.

Ordering Information

Product

Number of Units

Catalog Number

Microarray Microplate 384, with 384 round wells per plate, conical bottoms and polypropylene construction

50 per case

MMP384

Microarray Microplate Cover 384, clear plastic plate lids

50 per case

MMC384

Warranty
Arrayit's ArrayIt® Brand Microplates are sold for research purposes only. Arrayit's products are not for use in human diagnostics or for drug purposes, nor are they to be administered to humans in any way. Extreme care and exact attention should be practiced in the use of the materials described herein.  All Arrayit products are subject to extensive quality control and are guaranteed to perform as described when used properly.  Any problems with these products should be reported to Arrayit immediately.  Our liability is limited to the replacement of the product, or a full refund.  Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

 
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