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Microarray Imprinters for use with Microarray Slides


Create your own hydrophobic well patterns for numerous microarray applications. The Microarray Slide Imprinter is an easy and cost effective way to partition microarray slides and substrates with a hydrophobic pattern for microarray and other slide based applications. This durable instrument is ideal for high throughput microarray experiments where multiple assays need to be run on a single microarray substrate slide with minimal risk of contamination between wells.

The Microarray Substrate Imprinter is a unique substrate slide partitioning technology that empowers the researcher to create microarray “wells” on virtually any microarray substrate slide.

Gaskets and other approaches
Gaskets without adhesive may not seal completely if the hardware is not designed properly and over time gaskets can wear out. If you prefer a high quality gasket type system, click here. Gaskets with adhesive can leak if the adhesive is soluble in the solvents used in processing the microarray. Gaskets must be removed before scanning which can cause some types of microarray surface chemistries to peel off the surface. Hydrophobic markers such as PAP pens are tedious and difficult to apply, are soluble in certain solvents, and the resulting partitions are often inaccurate. Some manufactured barriers are expensive, must be ordered in quantity, and design changes can be difficult and time consuming to implement.

Number of microarray “wells” per substrate slide

Reaction volume per microarray well

Estimated number of microarray spots per well

12

40 µl

500

16

60 µl

750

24

60 µl

500

48

6-7 µl

48

192

1-1.5 µl

16

End users will appreciate the following:

  • imprint hydrophobic patterns onto any slide and surface (any design/pattern can be imprinted)
  • empowers high throughput sample processing
  • pays for itself quickly by amortizing surface chemistry cost over many microarray experiments on a single slide/substrate
  • patterns meet micro spotting pin spacing of all Stealth and 946 printheads and microarrayer programming
  • parallel loading of samples using multi-channel pipettes or automated pipetting liquid systems such as Oasis and others
  • does not contribute to background or light scattering in scanning for colorimetric, CCD and laser/pmt fluorescent microarray scanners
  • stays on throughout all microarray processing and detection steps
  • compatible with a wide variety of aqueous and solvent based buffers
  • works for both DNA, protein, carbohydrate, small molecule and other types of microarrays
  • easy to use, no setup or assembly required
  • fast, each slide only takes a few seconds to imprint
  • fun to use
  • works with any and all microarray surface chemistry including hydrogels and membrane based surfaces such as SuperNitro and SuperProtein
  • inexpensive, low cost to operate and own

How the Microarray Slide Imprinter works
The Microarray Slide Imprinter provides a fast and convenient way to define multiple “wells” on a single microscope slide size substrate to create separate regions for many assays on a single substrate. This is accomplished by imprinting a thin, inert, hydrophobic film patterned onto the surface of a substrate slide using of a stamp to create individual reaction “wells”.

Step 1.  Select stamp and make sure it is immersed in a bath of hydrophobic film polymer.


Step 2. A microscope slide size substrate is inserted into a cartridge above the bath and held accurately for consistent positioning. Microarray locations are consistent from slide to slide.

Step 3. A lever on the outside of the imprinter is pulled so that the “stamp” can touch off on the surface of the substrate, thus imprinting the desired hydrophobic barrier lines onto the substrate. Microarrays can be now be manufactured and processed.

Since the printed hydrophobic pattern printed is thin, the imprinted slides/substrates can be inserted directly into microarrayers and microarray processing racks and wash stations and directly into a microarray scanners without any further modifications. The coating is highly hydrophobic, does not dissolve in most solvent systems, and will stay on the slide through multiple wash steps and overnight incubations. The Microarray Slide Imprinter offers excellent flexibility.  The imprinted hydrophobic barriers can be made in any design and works with all microarray surface chemistries including membranes and hydrogels. Additionally the barrier shows excellent stability in most solvents through repeated washes, is chemically inert and does not contribute to light scatter or background noise in detection.

Image of a 12 well imprinted slide using food coloring for visual effect to show how the different areas of the slide will not cross contaminate.

Background light scattering comparison between imprinted microarray slide (left image) and a slide with common hydrophobic ink (right image) scanned with a typical laser/pmt microarray scanner.  Yellow border added for visualization of data.  Note that the imprinted pattern on the slide (left image) does not show up in the scan and therefore does not contribute to background noise nor does it scatter any signal.  Border on the ink printed slide shows completely saturated signals (right image).

Ordering Information

Product Description

Catalog ID

Microarray Substrate Slide Imprinter containing a 12 Microarray Stamp for creating 12 individual “wells” on standard glass microarray substrate slides.

MSI12

Microarray Substrate Slide Imprinter containing a 12 Microarray Stamp for creating 16 individual “wells” on standard glass microarray substrate slides.

MSI16

Microarray Substrate Slide Imprinter containing a 12 Microarray Stamp for creating 48 individual “wells” on standard glass microarray substrate slides.

MSI48

Microarray Substrate Slide Imprinter containing a 12 Microarray Stamp for creating 192 individual “wells” on standard glass microarray substrate slides.

MSI192

Custom Designed Microarray Substrate Slide Imprinter containing a custom Microarray Stamp for creating a custom number individual “wells” on standard glass microarray substrate slides. Customer to specify design parameters.

MSICUST

Replacement Stamp for creating 12 individual “wells” on standard glass substrate slide microarrays. For use with all Microarray Substrate Slide Imprinters.

STAMP12

Replacement Stamp for creating 16 individual “wells” on standard glass substrate slide microarrays. For use with all Microarray Substrate Slide Imprinters.

STAMP16

Replacement Stamp for creating 48 individual “wells” on standard glass substrate slide microarrays. For use with all Microarray Substrate Slide Imprinters.

STAMP48

Replacement Stamp for creating 192 individual “wells” on standard glass substrate slide microarrays. For use with all Microarray Substrate Slide Imprinters.

STAMP192

Custom Replacement Stamp for creating a custom number of “wells” per substrate slide. Please specify number of wells per substrate. All stamps are interchangeable.

Inquire

Hydrophobic polymer replacement Prowax for use with all Microarray Substrate Slide Imprinters, (350 grams).

PROWAX

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with an ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund.  Any misuse of this product including deviations from our protocols is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.