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NanoPrint™ Protein Edition Microarray Printers


NanoPrint™ Protein microarrayers are enterprise level linear drive systems that provide universal platforms for research and diagnostic microarray manufacturing. Systems are now available with 192 Style Pins and Printheads.


Figure 1. Shown is the NanoPrint™ Protein LM60 system with a capacity of three 384-well sample microplates printing onto 60 standard glass substrates.


Figure 2. Shown is the NanoPrint™ Protein LM210 system with a capacity of three 384-well sample microplates printing onto 210 standard glass substrates.

NanoPrint™ Protein Microarrayers are industrial-level platforms for all microarray manufacturing applications including DNA microarrays, protein microarrays, and other types of biomolecules. NanoPrint™ Protein systems enable the manufacture of microarrays utilizing ArrayIt® 192, Professional, 946 and Stealth Style Micro Spotting Pins. The NanoPrint™ Protein uses superior linear drive motion control technology and proprietary Warp1 controllers, and is compatible with all standard microarray surfaces made by ArrayIt® and other glass substrate slide open platform vendors. Systems are easily configured to print onto different types of surfaces including 96-well plates, plate-sized glass, and proprietary cartridges and cassettes by taking advantage of flexible and modular deck configurations and an easy to use software interface.  The Microarray Manager Software combines unparalleled power and simplicity in a graphical user interface.  Click here for a NanoPrint™ Protein Microarray Manager Software tutorial.

Rationale for the NanoPrint™ Protein
A starter pack of consumables to get you started printing your sensitive samples immediately includes Microarray Microplates, SuperEpoxy Substrates, SuperProtein Substrates, Protein Printing Buffer, and BlockIt Blocking Buffer.

  • Proteins have a number of levels of organization. This includes the primary structure, (amino acid sequence, held together by covalent bonding), the secondary structure (structures such as the beta-pleated sheets and that are held together primarily by hydrogen bonding), the tertiary structure (held together by hydrogen bonding and disulfide bonds) and the quaternary structure (which combines multiple amino acid sequences together by hydrogen, disulfide and other bonding).
  • Each one of these levels of organization is required for an active protein and required to print high quality protein microarrays that can detect protein interactions. In addition, intact epitope structure is a requirement for antibody binding.
  • Hydrogen bonding, the force that maintains the correct folding of proteins, has temperature-dependant labiality. This temperature-dependant change in structure results in protein denaturation and inactivation and destruction of proteins. For every 10 degrees Celsius, the rate of denaturation doubles.
  • The optimal temperature for proteins in the liquid phase is 4şC. For the last 50 years, protein biochemistry has been performed in the cold room. The Protein Edition NanoPrint™ reduces or eliminates the requirement to work in the cold while maintaining protein structure.
  • Another key factor that affects protein integrity is microbial contamination (bacteria and fungi). The metabolic rate of microbes is greatly reduced in the cold, which reduces microbial doubling time and sample degradation.


 Software features include

  • A Method Creation Wizard
  • User & version control management
  • Custom calibration of the slide and microplate positions
  • Complete sample tracking
  • Support of input and output data files
  • Custom microarray designs
  • Customizable speed profiles and wash protocols
  • Automatic Method Validation
  • Runtime sample and spotting views as well as a Simulation Mode
  • Easy to use graphical Re-Print Wizard

The high speed, high precision linear servo control system of the NanoPrint™ Protein produces superior instrument performance that is essentially free of friction, noise and thermal emission. Combined with the compact bench top design, user configurable worktable, humidity and particulate control, a host of available options, and the flexible and sophisticated software, NanoPrint™ Protein systems offer the complete solution to high performance microarray printing.

NanoPrint Protein Options

  • 48 or 192 Maximum Pin Configurations
  • Anti-vibration table
  • Peltier-driven microplate cooling
  • Microplate printing worktables
  • Custom worktables for proprietary printing cassettes and cartridges
  • Worktable conveyor
  • Microplate stackers with lid removal and barcode reader
  • Dehumidification
  • Extended service warranty

The value of the linear servo motor technology used on NanoPrint™ Protein Microarrayers

  • Nanometer scale positional accuracy
  • Quiet operation
  • No vibration
  • No heat generation
  • No motor dust
  • Superior Accuracy
  • Near maintenance-free operation
  • High speed
  • High load capacity
  • Zero sagging with loss of power
  • Safer and much more durable than stepper motors

Specifications

Software

Interface

Intuitive and Graphic Windows 2000 or Windows XP User Interface

User Management

Password protected access levels for User, Manager and Administrator

Data Files

Both import and export of data files compatible with all current microarray detection instruments

Version Control

Complete tracking of all methods, version and backups

Automatic Method Validation

Automatically validates for compatible method parameters

Sample and printing Views

Each available in real-time during operation

Method Creation Wizard

Only minimal information required to create microarray printing protocols

Simulation Mode

Pre-test print methods in software prior to running programs

Flexible

Microarray design, pin configuration, worktable layout, source and destination type, offsets, speed profiles, wash/dry parameters and pre-print locations

Hardware

Size (L x W x H, cm)

LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm)

Weight (kilograms)

LM60 (150 kg), LM210 (200 kg)

Positional resolution

500 nanometers using linear drive Warp1 control

Printing speed

48 spots per second

Destination

Microarray substrates, glass slides, microplates, custom

Printing technology

Arrayit Pro, 946 or Stealth pins and printheads

Number of pins

Configurable 1 to 48

Spot diameter

65 microns or larger to meet all applications

Minimum spot spacing

50 microns

Pre-printing

User definable

Wash/Dry station

Ultrasonic with 2 wash positions and a dry station

Number of microplates

Three standard 384-well, customizable

Number of substrates

60 substrate slides (LM60) or 210 substrate slides (LM210)

Number of microplates

15 96-well microplates (LM60) or 45 96-well microplates (LM210)

Microplate type

96-well or 384-well microplates

Microplate cooling

Cool 1-3 microplates, for protein microarray applications

Environmental control

Fully enclosed, HEPA filtration and user-defined humidity control

NanoPrint™ Protein Flexible Deck Configurations
Front left view of the NanoPrint™ Protein showing three microplates, the washdry module and the printhead and pins printing onto glass substrates. A 48-pin NanoPrint Protein printhead loaded with 48 946 Micro Spotting Pins is printing location 8 on the deck. The modular deck design and platen cassettes allow users to easily switch between glass substrates, microplates, wafers and other types of substrates. Click here to view 96-well microplate printing.
Accessory Control Module (ACM)
The ACM unit provides computer control for the washdry, humidity, and ultrasonication stations on the deck of the NanoPrint™ Protein. The ACM unit fits neatly beneath the laboratory bench or under the optional anti-vibration table available with the system. The ACM is extremely quiet and operates only as needed by the system. Humidity is maintained in a user-specified manner of ±1%. Wash buffers are stored in carboys in front of the ACM.
NanoPrint™ Protein Pin Cleaning Module
NanoPrint™ Protein microarrayers are each equipped with a station that provides pin washing, drying and sonication of 1-48 pins. The sonicator is automatically filled and emptied during the print run in a completely automated manner. Systems sensors prevent splashing and overflowing for pin and deck safety. Drying is accomplished by vacuum using a quiet but powerful ACM-controlled function.  The Pin Cleaning Module is rugged, durable and easy to maintain.
NanoPrint™ Protein ACM Connectivity
Easy connectivity between the ACM and the robot and accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by sensors that automatically maintain the levels set by the user.
NanoPrint™ Protein Humidity Sensing
Accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by the RH sensor that automatically maintain the levels set by the user.
NanoPrint™ Protein Microplate Peltier Cooling
NanoPrint™ Protein systems offer sophisticated microplate cooling via Peltier as an affordable and highly recommended option for users wishing to minimize sample evaporation during printing. Microplate cooling is also highly recommended for protein microarray applications to minimize protein denaturation and microbial growth in recombinant protein samples. The Peltier module fits directly beneath the microplate for highly efficient cooling while maintaining a low deck profile.
NanoPrint™ Protein Software
The extraordinary and highly intuitive graphical user interface provides users with software writing speed, power and flexibility. Shown here is a screen shot of  the interstitial printing software command that automatically directs the robot to deposit the printed spots into a “orange packing” configuration for dramatically increased microarray printing density.
48 and 192 Pin Configurations are now available. 192 Pin configuration allows the user to run from 1 to 192 pins at 2.25 mm center to center spacing to pickup samples from 1536 well plates. Standard deliverable is 48 Pins at 4.5mm centers for use with 384 well plates (96 well plates can also be configured).  NanoPrint software and unique Pin and Printhead design allows the end user to setup the pin tool any way that they like. Multiplex array processing systems, plates, biochips micro fluidics, and cartridges can all be easily configured.
Superior Z-Axis Resolution for Optimum Spot Morphology
The Z-axis encoder on the NanoPrint™ Protein reads at 250-nanometer resolution. Unlike belt and stepper motor driven Z-axis motion control, the NanoPrint™ Protein offers highly precise resolution, repeatability and computer control over the speed and acceleration settings to ensure optimal printing onto any surface using our patented contact printing technology. Optimal parameters are set at the factory but can be easily changed by the user for printing onto many different surfaces.


Figure 3.  The NanoPrint™ Protein deck is configured in a module manner, allowing different worktables to be inserted and removed from the deck for different printing applications such as glass substrates, microplates, and proprietary cassettes and cartridges.  The screenshot shows a worktable allowing printing into 15 microplates (96-well) for the NanoPrint™ Protein LM60.


Figure 4. Data showing the printing consistency of eight different SMP3B pins in a single print run using the NanoPrint Protein LM60. The data were provided courtesy of W.G. Shreffler  and L. Bardina at the Mount Sinai School of Medicine (New York, USA).


Figure 5. Shown is the NanoPrint™ Protein LM210 system configured in an ArrayIt® class 100 cleanroom. The entire deck is accessible via two doors that open and close independently for user convenience and safety. The top of the Accessory Control Module (ACM) is shown beneath the robot.


Figure 6. Shown is the NanoPrint™ Protein LM210 system configured in an ArrayIt® class 100 cleanroom. Microarray manufacturing parameters including the number of pins, substrates, spots, technical replicates, printing pattern, worktable, printing offsets, speed, acceleration, and many other technical aspects are completely controllable by the operator via an intuitive graphical user interface (GUI).  The NanoPrint™ Protein MicroarrayManager Software allows users to write completely customized printing routines in minutes. Worktables allow the user to switch between glass substrate slides and microplates using different worktables and software routines.


Figure 7. Shown is the NanoPrint™ Protein 48-pin printhead designed to hold up to 48 microarray printing pins of the Professional, 946 or Stealth series. The attachment bayonet allows the printhead to be attached and removed for cleaning easily and precisely, eliminating the need for system re-calibration.  System can also be configure to run 192 Style Pins and Printheads.


Figure 8. Shown is the point of a Professional Series 3 (Pro3) microarray printing pin. Samples load into the pin uptake channel by capillary action, and direct contact between the sample layer on the pin tip and the glass printing substrate results in the formation of a printed microarray spot. The NanoPrint™ Protein LM60 and LM210 systems control pin movements in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per second. A total of 48 microarray printing pins of any tip size from the professional, 946 or Stealth series may be used on our NanoPrint™ Protein systems.


Figure 9. Shown is the Worktable Workstation for the NanoPrint™ Protein LM60 and LM210 systems. The Worktable Workstation allows fully automated conveyor-based loading and unloading of glass slide substrate, microplate, and custom worktables from the NanoPrint™ Protein deck. With a capacity of 60 worktables, the WorkTable Workstation allows fully automated printing onto 1800 glass substrates, 300 96-well microplates, or 900 custom cassettes. The Worktable Workstation integrates directly into both the LM60 and LM210 without the need for hardware modifications. Printing onto the worktables in easily specified in software using the Microarray Manager.

Figure 10. NanoPrint LM210 Microarrayer with 42-Plate Stacker installed in a cold room for specialized protein microarray manufacturing applications. Photograph provided courtesy of Johns Hopkins University, Baltimore, MD.

Ordering Information

Product

Description

Catalog ID

NanoPrint™ Protein LM60 Microarrayer

NanoPrint™ Protein Microarrayer LM60 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit’s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 deck prints from three 384-well microplates using 48 pins onto 60 glass substrates.

LM60PRO

NanoPrint™ Protein LM210 Microarrayer

NanoPrint™ Protein Microarrayer LM210 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit’s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 deck prints from three 384-well microplates using 48 pins onto 210 glass substrates.

LM210PRO

NanoPrint™ Protein Pro3 Printing Pins

NanoPrint™ Protein LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional946 orStealth pin series. Pro3 pins are our most popular pin choice for users placing pin durability over upfront cost as Pro3 pins are rated at 10 million printing cycles.

Pro3

NanoPrint™ Protein 946MP3 Printing Pins

NanoPrint™ Protein LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional946 orStealth pin series. 946MP3 pins are a popular choice for many research and commercial laboratories.

946MP3

NanoPrint™ Protein Printhead

NanoPrint™ Protein LM60 and LM210 microarrayers are equipped with a 48-pin printhead designed with an attachment bayonet for easy attachment and removal.

NPSPH48

NanoPrint™ Protein Worktable Workstation

Conveyor-based worktable workstation for the NanoPrint™ Protein LM60 and LM210 microarrayers, allowing fully automated loading and unloading of slide, microplate and custom cassette worktables from the NanoPrint™ Protein deck.

NPA

NanoPrint™ Protein Anti-Vibration Table

Custom anti-vibration table for the NanoPrint™ Protein LM60 and LM210 microarrayer systems.

NPA

NanoPrint™ Protein Microplate Stacker 189

Microplate stacker holding 189 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the NanoPrint™ Protein LM60 and LM210 systems.

NPA

NanoPrint™ Protein Microplate Stacker 42

Microplate stacker holding 42 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the NanoPrint™ Protein LM60 and LM210 systems.

NPA

NanoPrint™ Protein Dehumidification Apparatus

Apparatus that permits dehumidification of the NanoPrint™ Protein printing chamber. Compatible with the LM60 and LM210 systems.

NPA

NanoPrint™ Protein Triple Microplate Cooling

Liquid cooling apparatus driven by a Peltier device for all three microplate deck positions. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers.

NPA

NanoPrint™ Protein Single Microplate Cooling

Peltier cooling apparatus for a single microplate deck position. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers.

NPA

NanoPrint™ Protein Microplate Worktables

Microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print directly into 15 96-well microplates (LM60) or 45 96-well microplates (LM210). Worktables are easily removed to allow switching between glass substrate slides and microplates.

NPA

NanoPrint™ Protein Custom Worktables

Custom microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print onto their own proprietary cassettes or cartridges. Worktables are easily removed to allow switching between glass substrate slides, microplates, and custom cassettes and cartridges.

NPA

NanoPrint™ Protein Extended Warranty

NanoPrint™ Protein LM60 and LM210 microarrayers include a one-year warranty upon purchase. Users may purchase an Extended Warranty in one-year increments at an affordable price. Pins and Printheads are not included in the warranty coverage.

NPW60
NPW210

Microarray Instrument Cleaner

Arrayit High-Performance Instrument Cleaner removes dust, particulates, fingerprints and other contaminants from NanoPrint™ microarrayers. Apply using pump nozzle and Cleanroom Wipes, 65 ml of 1X solution.

AIC


NanoPrint™ Protein microarrayers are configured individually for each customer based on the number and type of printing pins, deck configuration, and options.


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