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Microarrayers - NanoPrint™ Microarrayers


NanoPrint™ microarrayer printers are enterprise level linear drive systems that provide a universal platform for research and diagnostic microarray manufacturing for any biomolecule type. Arrayit these systems come configured with high throughput Professional, 946 and 192 Pin Printing Devices.  These printing devices are covered under US Patent 6,101,946 and require a license for diagnostic applications.


Figure 1. Shown is the NanoPrint™ LM60 system with a capacity of three 384-well sample microplates printing onto 60 standard glass slide substrates.  Worktables for printing into microplates, plate size glass and other micro-fluidic biochip formats are available.


Figure 2. Shown is the NanoPrint™ LM210 system with a capacity of three 384-well sample microplates printing onto 210 standard glass slide substrates.

NanoPrint™ Microarrayers are industrial-level platforms for all microarray manufacturing applications including DNA microarrays, protein microarrays, and other types of biomolecules.

NanoPrint™ systems enable the manufacture of microarrays utilizing ArrayIt® Professional, 946 and Stealth Style Micro Spotting Pins. The NanoPrint™ uses superior linear drive motion control technology and proprietary Warp1 controllers, and is compatible with all standard microarray surfaces made by ArrayIt® and other glass substrate slide open platform vendors. Systems are easily configured to print onto different types of surfaces including 96 and 384-well plates, plate-sized glass, and proprietary cartridges and cassettes by taking advantage of flexible and modular deck configurations and an easy to use software interface.  The Microarray Manager Software combines unparalleled power and simplicity in a graphical user interface.  Click here for a NanoPrint™ Microarray Manager Software tutorial.

Software features include:

  • A Method Creation Wizard
  • User & version control management
  • Custom calibration of the slide and microplate positions
  • Complete sample tracking
  • Support of input and output data files
  • Custom microarray designs
  • Customizable speed profiles and wash protocols
  • Automatic Method Validation
  • Runtime sample and spotting views as well as a Simulation Mode
  • Easy to use graphical Re-Print Wizard

The high speed, high precision linear servo control system of the NanoPrint™ produces superior instrument performance that is essentially free of friction, noise and thermal emission. Combined with the compact bench top design, user configurable worktable, humidity and particulate control, a host of available options, and the flexible and sophisticated software, NanoPrint™ systems offer the complete solution to high performance microarray printing.

NanoPrint Options:

  • 192 Pin Printhead (48 Pin Professional Printhead provided as standard)
  • Anti-vibration table
  • Peltier-driven microplate cooling
  • Microplate printing worktables
  • Custom worktables for proprietary printing cassettes, biochips and cartridges
  • Worktable conveyor
  • Microplate stackers, refrigerated with lid removal and barcode reader
  • Dehumidification
  • Extended service warranty

The value of the linear servo motor technology used on NanoPrint™ Microarrayers:

  • Nanometer scale positional accuracy
  • Quiet operation
  • No vibration
  • No heat generation
  • No motor dust
  • Superior Accuracy
  • Near maintenance-free operation
  • High speed
  • High load capacity
  • Zero "sagging" with loss of power
  • Safer and much more durable than stepper motors

Specifications

Software

Interface

Intuitive and Graphic Windows 2000 or Windows XP User Interface

User Management

Password protected access levels for User, Manager and Administrator

Data Files

Both import and export of data files compatible with all current microarray detection instruments

Version Control

Complete tracking of all methods, version and backups

Automatic Method Validation

Automatically validates for compatible method parameters

Sample and printing Views

Each available in real-time during operation

Method Creation Wizard

Only minimal information required to create microarray printing protocols

Simulation Mode

Pre-test print methods in software prior to running programs

Flexible

Microarray design, pin configuration, worktable layout, source and destination type, offsets, speed profiles, wash/dry parameters and pre-print locations

Hardware

Size (L x W x H, cm)

LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm)

Weight (kilograms)

LM60 (150 kg), LM210 (200 kg)

Positional resolution

500 nanometers using linear drive Warp1 control

Printing speed

48 spots per second or 192 Spots second using “192” Series Pins and Printheads.

Destination

Microarray substrates, glass slides, microplates, custom

Printing technology

Arrayit Pro, 946 or Stealth pins and printheads

Number of pins

Configurable 1 to 48 at 4.5 mm centers or 1 to 192 at 2.25mm

Spot diameter

65 microns or larger to meet all applications

Minimum spot spacing

50 microns

Pre-printing

User definable

Wash/Dry station

Ultrasonic with 2 wash positions and a dry station

Number of microplates

Three standard 384-well, customizable

Number of substrates

60 substrate slides (LM60) or 210 substrate slides (LM210)

Number of microplates

15 96-well microplates (LM60) or 45 96-well microplates (LM210)

Microplate type

96-well or 384-well microplates

Microplate cooling

Cool 1-3 microplates, for protein microarray applications, or use the refrigerated stacker for higher throughput and hands free automation.

Environmental control

Fully enclosed, HEPA filtration and user-defined humidity control

NanoPrint Flexible Deck Configurations:
Front left view of the NanoPrint™ showing three microplates, the wash/dry module and the printhead and pins printing onto glass substrates. A 48-pin NanoPrint printhead loaded with 48 946 Micro Spotting Pins is printing location 8 on the deck. The modular deck design and platen cassettes allow users to easily switch between glass substrates, microplates, wafers and other types of substrates.

Accessory Control Module (ACM):
The ACM unit provides computer control for the wash/dry, humidity, and ultrasonication stations on the deck of the NanoPrint™. The ACM unit fits neatly beneath the laboratory bench or under the optional anti-vibration table available with the system. The ACM is extremely quiet and operates only as needed by the system. Humidity is maintained in a user-specified manner of ±1%. Wash buffers are stored in carboys in front of the ACM.

 

NanoPrint™ Pin Cleaning Module:
NanoPrint™ microarrayers are each equipped with a station that provides pin washing, drying and sonication of 1-48 pins. The sonicator is automatically filled and emptied during the print run in a completely automated manner. Systems sensors prevent splashing and overflowing for pin and deck safety. Drying is accomplished by vacuum using a quiet but powerful ACM-controlled function.  The Pin Cleaning Module is rugged, durable and easy to maintain.


NanoPrint™ ACM Connectivity:
Easy connectivity between the ACM and the robot and accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by sensors that automatically maintain the levels set by the user.

NanoPrint™ Humidity Sensing:
Accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by the RH sensor that automatically maintain the levels set by the user.

NanoPrint™ Microplate Peltier Cooling:
NanoPrint™ systems offer sophisticated microplate cooling via Peltier as an affordable and highly recommended option for users wishing to minimize sample evaporation during printing. Microplate cooling is also highly recommended for protein microarray applications to minimize protein denaturation and microbial growth in recombinant protein samples. The Peltier module fits directly beneath the microplate for highly efficient cooling while maintaining a low deck profile.

 

NanoPrint™ Software:
The extraordinary and highly intuitive graphical user interface provides users with software writing speed, power and flexibility. Shown here is a screen shot of  the interstitial printing software command that automatically directs the robot to deposit the printed spots into a “orange packing” configuration for dramatically increased microarray printing density.

48 and 192 Pin Configurations are now available. 192 Pin configuration allows the user to run from 1 to 192 pins at 2.25 mm center to center spacing to pickup samples from 1536 well plates.  Standard deliverable is 48 Pins at 4.5mm centers for use with 384 well plates (96 well plates can also be configured). NanoPrint software and unique Pin and Printhead design allows the end user to setup the pin tool any way that they like. Multiplex array processing systems, plates, biochips micro fluidics, and cartridges can all be easily configured.
Superior Z-Axis Resolution for Optimum Spot Morphology:
The Z-axis encoder on the NanoPrint™ reads at 250-nanometer resolution. Unlike belt and stepper motor driven Z-axis motion control, the NanoPrint™ offers highly precise resolution, repeatability and computer control over the speed and acceleration settings to ensure optimal printing onto any surface using our patented contact printing technology. Optimal parameters are set at the factory but can be easily changed by the user for printing onto many different surfaces.

 

Figure 3.  The NanoPrint™ deck is configured in a module manner, allowing different worktables to be inserted and removed from the deck for different printing applications such as glass substrates, microplates, and proprietary cassettes and cartridges. The screenshot shows a worktable allowing printing into 15 microplates (96-well) for the NanoPrint™ LM60.


Figure 4. Data showing the printing consistency of eight different SMP3B pins in a single print run using the NanoPrint LM60. The data were provided courtesy of W.G. Shreffler and L. Bardina at the Mount Sinai School of Medicine (New York, USA).


Figure 5. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100 cleanroom. The entire deck is accessible via two doors that open and close independently for user convenience and safety. The top of the Accessory Control Module (ACM) is shown beneath the robot.


Figure 6. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100 cleanroom. Microarray manufacturing parameters including the number of pins, substrates, spots, technical replicates, printing pattern, worktable, printing offsets, speed, acceleration, and many other technical aspects are completely controllable by the operator via an intuitive graphical user interface (GUI).  The NanoPrint™ MicroarrayManager Software allows users to write completely customized printing routines in minutes. Worktables allow the user to switch between glass substrate slides and microplates using different worktables and software routines.


Figure 7. Shown is the NanoPrint™ 48-pin printhead designed to hold up to 48 microarray printing pins of the Professional, 946 or Stealth series. The attachment bayonet allows the printhead to be attached and removed for cleaning easily and precisely, eliminating the need for system re-calibration.


Figure 8. Shown is the point of a Professional Series 3 (Pro3) microarray printing pin. Samples load into the pin uptake channel by capillary action, and direct contact between the sample layer on the pin tip and the glass printing substrate results in the formation of a printed microarray spot. The NanoPrint™ LM60 and LM210 systems control pin movements in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per second. A total of 48 microarray printing pins of any tip size from the Professional, 946 or Stealth series may be used on our NanoPrint™ systems.


Figure 9. Shown is the Worktable Workstation for the NanoPrint™ LM60 and LM210 systems. The Worktable Workstation allows fully automated conveyor-based loading and unloading of glass slide substrate, microplate, and custom worktables from the NanoPrint™ deck. With a capacity of 60 worktables, the WorkTable Workstation allows fully automated printing onto 1800 glass substrates, 300 96-well microplates, or 900 custom cassettes. The Worktable Workstation integrates directly into both the LM60 and LM210 without the need for hardware modifications. Printing onto the worktables in easily specified in software using the Microarray Manager.

Ordering Information 

Product

Description

Catalog ID

NanoPrint™ LM60 Microarrayer

NanoPrint™ Microarrayer LM60 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit’s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 worktable prints from 384-well microplates using 48 pins onto 60 glass substrates.

LM60

NanoPrint™ LM210 Microarrayer

NanoPrint™ Microarrayer LM210 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit’s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 deck prints from three 384-well microplates using 48 pins onto 210 glass substrates.

LM210

NanoPrint™ Pro3 Printing Pins

NanoPrint™ LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional, 946 or Stealth pin series. Pro3 pins are our most popular pin choice for users placing pin durability over upfront cost as Pro3 pins are rated at 10 million printing cycles.

Pro3

NanoPrint™ 946MP3 Printing Pins

NanoPrint™ LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional, 946 or Stealth pin series. 946MP3 pins are a popular choice for many research and commercial laboratories.

946MP3

NanoPrint™ Printhead

NanoPrint™ LM60 and LM210 microarrayers are equipped with a 48-pin printhead designed with an attachment bayonet for easy attachment and removal.

NPSPH48

NanoPrint™ Worktable Workstation

Conveyor-based worktable workstation for the NanoPrint™ LM60 and LM210 microarrayers, allowing fully automated loading and unloading of slide, microplate and custom cassette worktables from the NanoPrint™ deck.

NPA

NanoPrint™ Anti-Vibration Table

Custom anti-vibration table for the NanoPrint™ LM60 and LM210 microarrayer systems.

NPA

NanoPrint™ Microplate Stacker 189

Microplate stacker holding 189 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the NanoPrint™ LM60 and LM210 systems.

NPA

NanoPrint™ Microplate Stacker 42

Microplate stacker holding 42 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the NanoPrint™ LM60 and LM210 systems.

NPA

NanoPrint™ Dehumidification Apparatus

Apparatus that permits dehumidification of the NanoPrint™ printing chamber. Compatible with the LM60 and LM210 systems.

NPA

NanoPrint™ Triple Microplate Cooling

Liquid cooling apparatus driven by a Peltier device for all three microplate deck positions. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers.

NPA

NanoPrint™ Single Microplate Cooling

Peltier cooling apparatus for a single microplate deck position. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers.

NPA

NanoPrint™ Microplate Worktables

Microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print directly into 15 96-well microplates (LM60) or 45 96-well microplates (LM210). Worktables are easily removed to allow switching between glass substrate slides and microplates.

NPA

NanoPrint™ Custom Worktables

Custom microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print onto their own proprietary cassettes or cartridges. Worktables are easily removed to allow switching between glass substrate slides, microplates, and custom cassettes and cartridges.

NPA

NanoPrint™ Extended Warranty

NanoPrint™ LM60 and LM210 microarrayers include a one-year warranty upon purchase. Users may purchase an Extended Warranty in one-year increments at an affordable price. Pins and Printheads are not included in the warranty coverage.

NPW60
NPW210

Microarray Instrument Cleaner

Arrayit High-Performance Instrument Cleaner removes dust, particulates, fingerprints and other contaminants from NanoPrint™ microarrayers. Apply using pump nozzle and Cleanroom Wipes, 65 ml of 1X solution.

AIC

NanoPrint™ microarrayers are configured individually for each customer based on the number and type of printing pins, deck configuration, and options.

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