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Environmental, Food and Herbal Medicine Allergy Microarray Tests and Testing Services

Allergy Test

Arrayit Allergen Microarrays Test and Screen for 123 Allergens for Immunoglobulin E (IgE) and 101 Allergens for Immunoglobulin G (IgG) present in Human Serum. We offer IgE and IgG allergen microarrays for use in your laboratory and as a complete service. These products are for Research Use Only.

Product Advantages

  • Screens the most common environmental, food and herbal medicine allergens
  • Easy to understand reports contain user-friendly charts
  • World-leading Arrayit Microarray Platform
  • Protein Microarray Technology offers best performance

The foundation of the test is Arrayit’s Protein Microarray Platform. Specially prepared allergens to optimized antibody recognition as probe biomarkers are “nano spotted” and covalently bound on the surface of a glass slide in a microarray format using Arrayit’s patented microarray manufacturing technology. To perform the assay, a serum sample is added to the microarray of allergens creating an antigen-antibody binding reaction. A secondary antibody with fluorescent dye is then added to the microarray allowing for specific wavelengths of a confocal microarray laser scanner to pin-point the specific antibodies inducing allergic reactions.

Allergy Testing

Figure 1. Allergies and Immune Response can be measured using the high-specificity, high-sensitivity Arrayit protein microarray platform.

An allergic reaction occurs when the immune system overreacts to something, often in the environment but can also be food or a herbal medicine that is harmless to most people. To protect the body from this perceived threat, or allergen, the immune system of an allergic person produces antibodies called immunoglobulins.

Immunoglobulin antibodies are found mostly in the lungs, skin, and mucous membranes and can be measured by a blood test. They cause mast cells (a type of cell involved in the body's immune response) to release chemicals, including histamine, into the bloodstream. It is these chemicals that bring on many of the allergy symptoms that affect a person's eyes, nose, throat, lungs, skin, or gastrointestinal tract.

Because IgE and IgG antibodies are unique to each allergen, checking for specific variants in the blood can help determine if an allergy is present.

Acute allergy measured by Immunoglobulin E Antibodies. Asthma and urticaria (rashes, redness, inflammation of skin), and shortness of breath are the most common clinical signs. The eyes, nose, throat, lungs, skin, or gastrointestinal tract can all be adversely affected by allergic reactions. Generally, allergic can be improved by determining and avoiding trigger allergens identified by an IgE/IgG test.

Chronic allergy is measured by Immunoglobulin G Antibodies. This type of allergy is mostly caused by foods. However, slow onset and non-specific symptoms make it difficult to make a correct identification of the allergen and this is where microarray data from this test can valuable.

Microarray-Elisa

Figure 2. Example image from a typical 2-color microarray scan.  Red, yellow and green spots are displayed on the computer screen.  Red spots have signal only in red channel, yellow spots have equal signals in both channels, green spots have signal only in green channel.  Signal intensity measuring IgE (red) and IgG(green) is done by a confocal laser microarray scanner that digitizes the signal into numerical values to quantify and report the amount of IgE and IgG binding by the allergen microarray.  In this example each allergen appears on the microarray as two technical replicates.

Microarray Advantages:

Compared to the traditional ELISA (Enzyme-linked immune-sorbent assay) allergy analysis, our allergy testing platform is facilitated by innovative automatic scanning and analysis software to test many allergens and controls in a signal assay.

Minimal serum amount tests many allergens: Lower serum volume required than traditional ELISA assays while testing many allergens with positive and negative controls in a single reaction.

Stability and sensitivity: We conduct triplicate tests on each microarray to ensure the stability of each test.   At the same time, we can detect the concentration limits.

Environmentally-friendly and cost effective: Small amount of reagents and consumables are used to perform the test, therefore lowering the consumption of resources.

High efficiency: Multiple samples can be conducted simultaneously over many allergens and the results are available quickly. Test contains the highest number allergens available testing for IgE in a single assay.

High Sensitivity : Fluorescent detection coupled with a highly miniaturized assay enables the most sensitive assay available to detect Immunoglobulins E and G.

High Accuracy: Test allergens and controls are all performed simultaneously in the same microarray reaction.

123 IgE Allergens List


Mold/Pollen
Cladosporium herbarum     Alternaria alternate     Aspergillus fumigatus     Penicillium notatum     Candida albicans     Junegrass(Ky Blue)     Bermuda     Sorrel(red)     Ragweed(short)     Pigweed(rough/redroot)     Timothy     Acacia     Mulberry(paper)     Pine(Australian)

Mite/Dander
D.pteronyssinus     D.farinae     B.tropicalis     Cockroach     Dog epithelia     Cat hair     Goat epithelia

Feather mix

Dairy Products
Milk     Cheese     Yogurt     Egg white     Egg yolk

Grains/Nuts
Rice     Wheat     Buck wheat     Corn     Soybean     Mung bean      Red bean     Runner bean     Peanut     Cashew nut     Walnut     Sesame seed     Sunflower seed     Almond    Olive

Meats
Pork      Beef     Lamb     Chicken      Duck      Goose

Seafoods
Shrimp     Crab     Clam     Oyster     Squid     Cod fish     Salmon     Tuna      Eel     Seaweed

Vegetables
Spinach      Cabbage     Lettuce     Celery      Broccoli     String bean      Peas     Green pepper     Eggplant      Pumpkin      Cucumber      Asparagus     Bamboo shoots     Radish     Carrot     Sweet Potato      White Potato      Taro     Tomato     Mushroom     Onion

Fruits
Mango     Kiwi      Apple     Banana     Pineapple     Cherry      Grape      Grape fruit     Citrus      Guava     Strawberry      Lemon     Honey peach      Coconut     Cantaloupe     Papaya     Watermelon     Pear

Yeast/ Beverages
Yeast     Tea     Coffee     Cocoa bean     Honey

Spices
Scallion     Ginger      Garlic      Curry     Chili      White pepper     Basil     Coriander

Herbal Medicines
Lycium     Astragalus     Red date     Licorice     Angelica      Ginseng     Cindium     Rehmannia     Longan pulp     Snow fungus     Lotus seed     Reishi      Codyceps

101 IgG Allergens List


Dairy Products
Milk     Cheese     Yogurt     Egg white     Egg yolk

Grains/Nuts
Rice     Wheat      Buck wheat     Corn    Soybean    Mung bean    Red bean    Runner bean    Peanut    Cashew nut    Walnut    Sesame seed    Sunflower seed    Almond    Olive

Meats
Pork    Beef    Lamb    Chicken    Duck    Goose

Seafoods
Shrimp   Crab   Clam   Oyster     Squid     Codfish     Salmon     Tuna     Eel     Seaweed

Vegetables
Spinach     Cabbage     Lettuce     Celery     Broccoli     String bean     Peas     Green pepper     Eggplant     Pumpkin     Cucumber     Asparagus   Bamboo shoots     Radish     Carrot     Sweet Potato      Potato     Taro     Tomato     Mushroom     Onion

Fruits
Mango     Kiwi     Apple     Banana     Pineapple     Cherry     Grape     Grapefruit     Citrus     Guava     Strawberry     Lemon     Honey peach     Coconut     Cantaloupe     Papaya     Watermelon     Pear

Yeast/ Beverages
Yeast     Tea     Coffee     Cocoa bean     Honey

Spices
Scallion     Ginger     Garlic     Curry     Chili     White pepper     Basil     Coriander

Herbal Medicines
Lycium     Astragalus     Red date      Licorice     Angelica     Ginseng     Cindium     Rehmannia     Longan pulp     Snow fungus     Lotus seed     Reishi     Codyceps

 

Ordering Information

Product

Description

Catalog ID

Allergen Microarray Tests

Arrayit protein microarray allergy tests including environmental, food and herbal medicine allergens printed as protein microarrays on standard glass substrate slides for IgE (123 allergens) and IgG (101 allergens) testing in your laboratory. These 1 x 25 x 76 mm microarray slide tests are compatible with all glass slide-based microarray scanners and other microarray equipment, pricing is per microarray, for research use only.

AMT

Allergen Microarray Testing Service Arrayit protein microarray allergy testing service including blood card sampling kit, sample processing, and complete test report for each sample tested. Our serum-based microarray assay measures acute and chronic allergic reactions to 123 IgE allergens and 101 IgG allergens.  The test report is delivered as a PDF document for each serum sample. Maximum lead time is 5 business days from receipt of sample, pricing is per sample, for research use only. AMS