Home AsterScan Multi-modal Scanning system High throughput and high resolution multiple channels system

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AsterScan® Multi-modal Microarray Scanner

 

AsterScan Microarray Scanner.

 

 

 

FLUORESCENCE AND COLORIMETRY

FAST and HIGH-RESOLUTION SCANS 

VARIOUS SUBSTRATES

VERY HIGH THROUGHPUT

 

 

- Overview

- Imaging

- Microarray Analysis

- Documentation

-Technical Specifications

While linear scanning systems are progressively reaching the limits of the technology for scanning microarrays, imaging systems for cells and tissues analysis are persistently developing powerful fluorescence microscopes, which allow to cross over many unexpected borders once applied in the field of microarrays analysis.

Based on a powerful embedded inverted microscope, working in bright field and in fluorescence, AsterScan® utilizes a high precision motorized stage to scan your samples. Equipped with high resolution CCD cameras with Peltier cooling, AsterScan® allows you to scan your microarrays as well as cells and tissues with unmatched speed and precision. It’s optical resolution range covers pixel sizes from 3.2 to 0.2 µm/pixel. An integrated stacker allows you to work with an impressive number of slides hence increasing your throughput. An attached Joystick allows you to navigate live on your samples for manual verification and control.


Figure 1. Shown is the AsterScan® with a  rack of samples removed from the stacker to allow to see the imaging part of the instrument.

AsterScan Lateral Imaging part AsterScan Imaging part

Figure 2. Shown is the Joystick and screenshots of the two large screens used for live navigation on the samples.

AsterScan Joystick

AsterScan Screenshots

Overview

An impressive stacker with 60 positions for SBS - footprint samples is feeding the imaging part installed on the right of the instrument, thanks to a central axis equipped with a gripper and a barcode scanner for samples detection and identification.

Samples are placed on a high precision motorized stage into the embedded inverted microscope, to provide, thanks to a sophisticated imaging software package, microscopic live imaging and documentation by using bright field as well as fluorescence illumination. AsterScan® offers a Software controlled external automated filter wheel for up to six fluorescence excitation filters to enable the detection of various fluorochroms.

The application range reaches from scanning and analysis of microarrays into live cell imaging of cell culture plates and dishes, and other measurement tasks.

The users will appreciate the following features :

Application : Scanning of Microarrays and Cell imaging on various substrates, easy zoom out for inspection & analysis (e.g.: 10 µm)
Sample Carriers : - Micro-titer Plates with SBS format, 1 to 1536 well plates

- Microarray slides 76x26mm or custom, using adapters for 3 or 4 slides

Multi-modal : Scans in colorimetric (brightfield) and fluorescence mode (Cy3, Cy5, DAPI, FITC, TRITC, mCherry, mKate2, Alexa Fluor 594, Texas red and many more) with simultaneous or sequential readout of multiple wavelengths.
Speed : Scans 4 microarray slides or 1 four-well rectangular compartment plate in 10 minutes, one 96-well plate in 14 minutes (at 3.2 µm pixel size)
Acuracy : pixel size from 3.2 µm to 0.2 µm, easy zoom out for inspection & analysis (e.g.: 10 µm)
Calibration and Revision : Visual inspection by live navigation driven with a joystick, with direct feedback on adjustments of settings
Imaging and Detection : - Powerful software with sophisticated algorithms to guarantee a successful recognition and detection of features of interest

- Plethora of morphology and spectral parameters as well as grey intensities

User Interface : user-friendly GUI for easy analysis of microarrays, import of GAL files, subtraction of local background and reporting functions with a complete record for tracking of samples
Throughput : - high precision integrated X/Y motorized stage with SBS-plate footprint holder; adapters for 3 to 4 microarray slides; custom adapters available

- integrated stacking system with barcode reader and plate detection for up to 60 micro well plates or 60x4 microarray slides. Automated imaging and analysis.

NB : system available without stacker

Figure 3 : AsterScan® available without stacking system (shown here without enclosure, Code ASC-LT) :

AsterScan without stacker

The Imaging

Each scanning process runs according to predefined settings to guarantee a successful recognition and detection of the microarrays or cells of interest. These settings can be easily adopted by the user and include a plethora of morphology (figure 4) and spectral parameters as well as grey intensities (figure2).

Figure 4 : Measurements Parameters

Measurements Parameters

Focus can be set manually, and automated on each of the samples scanned, based on as many points as assigned by the user.

A lateral door permit easy adjustments of magnification by changing manually the objectives (figure 5), leading to image resolutions from 3.2 to 0.2 µm/pixel ! A frontal small door gives access to a manual control of the white light power.

 

Figure 5 : Objective wheel

Objective door

AsterScan Objectives

AsterScan® 'software allows to scan many different fluorescence dyes simultaneously or sequentially, and to setup many parameters for automation.

 

Fig 6 : Microarrays can be imaged onto traditional glass slides, with transparent or nitrocellulose coating, and onto various micro-well plates with SBS-format. Custom formats possible.

AsterScan Slide Fluo Nitro Green AsterScan Slide Fluo Nitro Pink AsterScan 96 Wells AsterScan 4 wells


Microarray Analysis

Morphology, distances and spot sizes can be inspected easily on the images (Figure 7) :

AsterScan Slide Selection AsterScan Slide Selection AsterScan Array Inspection

GAL files imported will assign automatically the area of scanning and will apply adequate grids onto the arrays. Various powerful algorithms can be choosen by the user for spot detection and backgroung noise acquisition around each spot, so that signals from perfect round spots or from spots in "sky" format made for example by cells transfected onto microarrays are perfectly analyzed (Figure 8) :

AsterScan Array Inspection AsterScan Transfected Cells AsterScan Analysis Area Zoom in

Documentation

The informaton provided with the barcodes are integrated together with the GAL files in a complete documentation file, with mean and median values for the spots, background values etc...
The entire process is documented via real-time images and the numeric data including all user defined detection parameters such as morphology or spectral values are saved in Excel compatible datasheets.

A user-friendly Documentation Mode allows the user to view the entire experiment including images and data files at once (figure 9) :

AsterScan Documentation Mode

Specifications :

Optical Resolution 0.26 – 3.2 µm/pixel
Excitation wavelength Up to 6 free configurable excitation wavelengths
Compatible fluorophores All fluorophores compatible with configured filter sets

Compatible slides

Standard microscope slides

SBS based well plates (1 well to 1536-Well-Plate)

Custom plates, slides and chips
Maximal scan area 125 x 85mm
Focus Automatic (3 different modes) or manual

Detection

Microscope image based detection through CCD Chip

High-Sensitivity Mode 2x, 4x and 8x

Gain

Image Scaling feature in Software

Binning 2x2; 4x4; 8x8
Scanning mode Simultaneous or sequential
Scanning speed
3 - 10 images/sec.
Scanning time 2.5 minutes to scan a whole slide at a 3.2 µm/pixel resolution
Dimensions 100 W x 70 D x 100 H cm
Weight 120 kg
Interface Ethernet
Barcode scanner Yes
Image processing software AsterScan Software Package
Image file format 16-bit and 24-bit TIFF

Ordering Information

Product

Description

Catalog ID

AsterScan®

coming soon...

ASC

AsterScan® Low throughput

coming soon...

ASC-LT

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