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Microarray Tools - Multi-Well Microarrays

Hybridization Multiwells

ArrayIt® offers an innovative hardware solution for multi-well microarrays. Pictured above is catalog ID: AHC4x8. A silicone gasket containing 8, 16 or 24, 48 or 96 wells is held against the glass surface to create individual reaction wells encompassing the printed microarrays. The upper portion of the Hybridization Cassette allows the introduction of individual samples, making these Cassettes, ideal for DNA and protein microarray research and diagnostics. NOTE! It is possible to cut the ribs of these gaskets to make larger size reaction areas!  Easily sealed with foil seals to prevent evaporation.

Figure 1A.The AHC1X24 has 7.5 x 6.5 mm wells spaced at 9 mm centers in a 3 x 8 format for easy loading with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the gasket against the hardware and the thin portion against the glass substrate slide.

Clic here to see the video

Figure 1B.The AHC1X16 has 7.5 x 7.5 mm wells spaced at 9 mm centers in a 2 x 8 format for easy loading with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the gasket against the hardware and the thin portion against the glass substrate slide.



Figure 2. Diagramatic depiction of the wells formed by Hybridization Cassette 1x24 (left) and 1x16 (right). Use these spacing coordinates to program the microarrayer prior to printing the 3x8 (1x24) or 2x8 (1x16) microarray patterns required to utilize the multi-well Hybridization Cassettes. Use the SpotBot and NanoPrint Microarrayer to simplify the microarray manufacturing using these formats. NOTE! It is possible to cut the ribs in these gaskets to make larger size reaction areas!


Figure 3. Hybridization Cassette 4x16 (Catalog ID AHC4X16) is identical to Hybridization Cassette 1x16 except that it contains four identical units allowing the use of four glass substrate slide microarrays. The Hybridization Cassette 4x16 has a 7.5 x 7.5 mm well size set at 9 mm centers in four identical 2x8 units. Well openings in the top portion of the Hybridization Cassette (shown here) are 5.5 x 5.5 mm. Requires the use of four individual silicon gaskets (Catalog ID GAHC1x16) to demarcate the individual wells, and the four gaskets are included with the purchase of the AHC4x16 product.

Hyb Cassette 4x24
Figure 4. Hybridization Cassette 4x24 (Catalog ID AHC4X24) is identical to Hybridization Cassette 1x24 except that it contains four identical units allowing the use of four glass substrate slide microarrays. The Hybridization Cassette 4x24 has a 7.5 x 6.5 mm well size set at 9 mm centers in four identical 3x8 units. Well openings in the top portion of the Hybridization Cassette (shown here) are 5.5 x 5.5 mm. Requires the use of four individual silicon gaskets (Catalog ID GAHC1x24) to demarcate the individual wells, and the four gaskets are included with the purchase of the AHC4x24 product.


Figure 5. Silicone Replacement Gaskets for multi-well microarray Hybridization Cassettes. Use Replacement Gasket GAHC1x16 (upper left) with Hybridization Cassette AHC1x16, Replacement Gasket GAHC1x24 (upper right) with Hybridization Cassette AHC1x24, Replacement Gasket GAHC1x16 four each (lower left) with Hybridization Cassette AHC4x16, and Replacement Gasket GAHC4x24 (lower right) with Hybridization Cassette AHC4x24. To prevent leakage during use, gaskets must be used in the proper orientation with the wide portion placed against the hybridization cassette top and the thin portion placed against the glass substrate slide. NOTE! It is possible to cut the ribs in these gaskets to make larger size reaction areas!

Format Hyb Cassette


Figure 6. Format for Catalog ID: AHC4x8S Arrayt Hybridization Cassette 4 slides 8 Microarrays per slide substrate. For larger wells use catalog ID: AHC4x8L. There are 8 rectangular shapped wells at 9 mm center to center spacing down the slide substrate.

Format L Hyb Cassette

Figure 7. Format for Catalog ID: AHC4x8L Arrayt Hybridization Cassette 4 slides 8 Microarrays per slide. There are 8 rectangular shapped wells at 9 mm center to center spacing down the slide substrate

Hybridization Station


Figure 8. Array Plate Multi-Well Hybridization Stations are available for 4x8, 4x16 and 4x24 Hybridization Cassettes. Click here for product details. Reaction tool easily sealed with foil seals to prevent evaporation during long reactions.  Wash using Microarray Wash Trays and a Plate Shake
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Recommended Equipment and Reagents
NanoPrint Microarrayers
SpotBot® 2 Personal Microarrayers
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuge
Protein Printing Buffer
BlockIt Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers

Ordering Information

Product

Description

Catalog ID

Hybridization Cassette, 1x16

ArrayIt® multi-well Hybridization Cassette containing a base, 16-well silicon gasket and a top portion, 16 7.5 x 7.5 mm wells in a 2x8 pattern at 9 mm centers, single glass substrate slide microarray capacity.

AHC1X16

Hybridization Cassette, 1x24

ArrayIt® multi-well Hybridization Cassette containing a base, 24-well silicon gasket and a top portion, 24 7.5 x 6.5 mm wells in a 3x8 pattern at 9 mm centers, single glass substrate slide microarray capacity.

AHC1X24

Hybridization Cassette, 4x16

ArrayIt® multi-well Hybridization Cassette containing a base, four 16-well silicon gaskets and a top portion, 64 7.5 x 7.5 mm wells in four 2x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.

AHC4X16

Hybridization Cassette, 4x24

ArrayIt® multi-well Hybridization Cassette containing a base, four 24-well silicon gaskets and a top portion, 96 7.5 x 6.5 mm wells in four 3x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.

AHC4X24

Hybridization Cassette, 4x8 Small

ArrayIt® multi-well Hybridization Cassette containing a base, 4x8-well silicon gaskets and a top portion, 48, 8 x 17 mm wells in four 1x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.

AHC4X8S

Hybridization Cassette, 4x8 Large

ArrayIt® multi-well Hybridization Cassette containing a base, 4x8-well silicon gaskets and a top portion, 48, 8 x 23 mm wells in four 1x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.

AHC4X8L

Replacement Gaskets GAHC1x16

Silicon Replacement Gasket for the ArrayIt® multi-well Hybridization Cassettes AHC1x16 and AHC4x16, allowing the formation of 16 7.5 x 7.5 mm wells in a 2x8 pattern at 9 mm centers. Use 1 each for AHC1x16 and four each for AHC4x16.

GAHC1X16

Replacement Gaskets AHC1x24

Silicon Replacement Gasket for the ArrayIt® multi-well Hybridization Cassette AHC1x24, allowing the formation of 24 7.5 x 6.5 mm wells in a 3x8 pattern at 9 mm centers.

GAHC1X24

Replacement Gaskets 4x16

Silicon Replacement Gaskets for the Arrayit multi-well Hybridization Cassette AHC1x16, 4 gaskets per set.

GAHC4X16

Replacement Gaskets 4x24

Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.

GAHC4X24

Replacement Gaskets 4x8S

Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.

GAHC4X8S

Replacement Gaskets 4x8L

Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.

GAHC4X8L

Microarray Wash Tray

Convenient wash tray to use with multi-well hybridization cassettes.

MWT

Microarray Hybridization Station

Convenient instrument to control temperature and mix with these hybridization multi-well hybridization cassettes

MMHS110V and MMHS220V

 

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

 
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