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Whole Cell Microarray Printing Pins


Arrayit Whole Cell Microarray Printing Pins leverage the most widely used microarray manufacturing technology in the world, with 100 µm and 500 µm wide uptake channels and 1.25 µl loading volumes to efficiently print intact human, fungal and bacterial cells into microarrays. Whole cell microarray printing pins are compatible with Professional, 946 and Stealth style Printheads NanoPrint™ LM60, LM210, SpotBot® 3, SpotBot® Extreme and SpotBot® Titan Microarrayers. Whole cell pins can also be used to print microarrays containing 500-750 µm spots and 200 nl spot volumes.

Reference Publication:
Human Cell Chips: Adapting DNA Microarray Spotting Technology to Cell-Based Imaging Assays
T Hart, A Zhao, A Garg, S Bolusani, EM Marcotte - PLoS ONE, 2009 - dx.plos.org


Abstract: Here we describe human spotted cell chips, a technology for determining cellular state across arrays of cells subjected to chemical or genetic perturbation. Cells are grown and treated under standard tissue culture conditions before being fixed and printed onto replicate glass slides, effectively decoupling the experimental conditions from the assay technique. Each slide is then probed using immunofluorescence or other optical reporter and assayed by automated microscopy. We show potential applications of the cell chip by assaying HeLa and A549 samples for changes in target protein abundance (of the dsRNA-activated protein kinase PKR), subcellular localization (nuclear translocation of NFκB) and activation state (phosphorylation of STAT1 and of the p38 and JNK stress kinases) in response to treatment by several chemical effectors (anisomycin, TNFα, and interferon), and we demonstrate scalability by printing a chip with ~4,700 discrete samples of HeLa cells. Coupling this technology to high-throughput methods for culturing and treating cell lines could enable researchers to examine the impact of exogenous effectors on the same population of experimentally treated cells across multiple reporter targets potentially representing a variety of molecular systems, thus producing a highly multiplexed dataset with minimized experimental variance and at reduced reagent cost compared to alternative techniques. The ability to prepare and store chips also allows researchers to follow up on observations gleaned from initial screens with maximal repeatability.


Figure 1. Whole Cell Microarray Printing Pin (Cat. WCMPL) tip at 40X magnification, showing the flat 500 µm wide tip and uptake channel. WCMPL whole cell pins are used to manufacture whole cell microarrays and printed microarrays of 500- 750 µm diameter microarray spots and 200 nl per spot printed volume.

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License
This product is protected by US Patent Number 6,101,946 and is sold license- and royalty-free for research purposes only. Diagnostic applications require a license, contact us for more information about licensing.
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Ordering Information

Product

Cat.

Spot Diameter (µm)

Delivery Volume (nl)

Number of Spots per Load*

Min. Spot Spacing (µm)

Max. Subgrid per Pin 4.5 mm Spacing (spots)

Whole Cell Microarray Printing Pin with standard 100 µm sample channel

WCMP

220

1.5

600

255

17 x 17

Whole Cell Microarray Printing Pin with large (L) 500 µm sample channel

WCMPL

700-900

15-25

50

900

5 x 5

*Based on print tests using the NanoPrint Microarrayer at 50% humidity onto SuperEpoxy 2 microarray substrates.  Number of actual spots can depend on surface chemistry, buffer system and sample type. It is best to empirically test the number of reliable spots in the specific test system being used.

Warranty
ArrayIt® Brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® Brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® Brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product including the use of the wrong protocols or procedures is the full responsibility of the user.