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easy-spin llp Plant RNA Extraction Kit (Maxi-prep)

Ordering Information
Cat No. Size
17311 12 col.


Price

364.00 €

Add to Cart

PROTOCOL

Description

easy-spin IIp Plant RNA Extraction Kit is recommended for use total RNA isolation as maxi-scale from plant tissues. One of the kit component contains high molecular polyethyleneglycol (HMW-PEG) that binds to contaminants such as polyphenolic compounds and polysaccharides that are commonly present in plant tissues.
Glass fiber filter-based RNA isolation method, such as the easy-spinʂ IIp Plant RNA Extraction Kit, are especially suited for plant RNA purification because they do not include any alcoholic precipitating steps; many kind of contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation. When use with the easy-spinʂ IIp Plant RNA Extraction Kit, the procedure consists of disrupting the plant tissue in a guanidinium based solution to which the pre-lysis buffer has been added. A brief centrifugation then remove the polyphenolic and polysaccharide contaminants. The supernatant is passed through a glass-fiber filter under conditions that support RNA binding to glass filter. The filter is then washed to remove other contaminants and the RNA is recover in adequate volume (50 ~ 100 ul or more) of elution buffer.
The most remarkable characteristics of the easy-spinʂ IIp Plant RNA Extraction Kit is: (1) there is no genomic DNA contamination, (2) there is no alcohol PPT process, and (3) there is possible to recover a high quality of plant total RNA.

Characteristics

1. Extract total RNA from all kinds of plant tissue with easy and convenient.
2. Remove completely any cause for contaminants in the plant tissue.
3. Remove contamination of genomic DNA
4. Very convenient without procedure of Alcohol PPT
5. Guarantee High purity, High yield


Applicated Area

1. Extract plant pathogenic virus
2. Extract totla RNA from plant seed
3. Extract total RNA and synthesize cDNA from all kinds of plant

Kit Contents

  • Pre-lysis buffer† 50 ml
  • Lysis buffer† 100 ml
  • Binding buffer† 120 ml
  • Washing buffer A† 24 ml
  • Washing buffer B† 8 ml
  • : Ensure that 32 ml of EtOH is added to Washing buffer B before use.
  • Elution buffer† 30 ml
  • Pre spin column† 12 col.
  • Binding spin column† 12 col.
  • Collection tube† 12 tubes

Storage

Lysis Buffer : Store at 4°… after receiving
Other components: Store at Room temperature

Experiment Data

1) Data of extracting totla RNA from four different kinds of seed sample


iNtRON's easy-spinTM IIp shows much higher yield than company Q product.

2) Test virus detection infected by Seed sample


Extract total RNA from CGMMV-infected seed, then test virus detection by RT-PCR. iNtRON's easy-spinTM IIp shows higher sensitivity than A and Q company product.

easy-spin IIp Plant RNA Extraction Kit (Maxi-prep)
Ordering Information | Cat No 17311 Size 12 col.
364.00 €
Add to Cart

 
Instruments & Hardware
Pipettors Xiril