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Muta-Direct Site Directed Mutagenesis Kit
Ordering Information
Cat No Size
15071 15 rxn.


Price

203.00 €

Add to Cart

 

PROTOCOL

Description

Muta-Direct¢â Site-Directed Mutagenesis Kit can be used for mutagenesis. 

Laborious steps are not required such as M13 vector and methylation. 

It shows 100% efficiency and it takes only 2 days for all steps without any special skill.

Diagram of mutagenesis usig Muta-Direct Kit

Characteristics

  • Not required great experimental skill
  • Induction of the point mutation(s) within 2 days
  • Easy steps with only two enzyme : Muta-Direct¢â and MutazymeTM
  • Various application for point mutation, deletion, and insertion, etc.
  • Mutagenesis with 100% efficiency
  • Reasonable price

Storage

All components should be stored at -20¡ÆC.
Do not substitute provided buffers and dNTP mixture with others.

Kit Contents

Component
Quantity
Muta-Direct¢â> Enzyme (2.5 U/§¡)
15 §¡
Muta-Direct¢â Reaction Buffer (10 X)
100 §¡
dNTP Mixture

30 §¡

Mutazyme¢â> Enzyme (10 U/§¡)

15 §¡

pUC18 Control Plasmid (10 ng/§¡)

10 §¡

Control Primer Mix (20 pmol/§¡)
15 §¡
Competent Cells
Not provided

Example of experimental result

[PCR condition]

10X reaction buffer
5 §¡
Plasmid (6.3 kb, 10 ng/§¡)
2 §¡
Forward primer (10 pmol/§¡)
1 §¡
Reverse primer (10 pmol/§¡)
1 §¡
dNTP mixture (2.5 mM each)
2 §¡
Distilled water
38 §¡
Muta-Direct¢â Enzyme
1 §¡

Muta-Direct Site Directed Mutagenesis Kit
Ordering Information | Cat No 15071| Size 15 rxn.
203.00 €
Add to Cart

 
Instruments & Hardware
Pipettors Xiril