Home Klenow Fragment 10U/ul

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Klenow Fragment
Ordering Information
Cat No Size
27042 300U/§¡


Price

145.00 €

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PROTOCOL

Description
Klenow fragment process 3' to 5' exonuclease activity but lack the 5' to 3' exonuclease activity. And it is suited for random-primed labeling of DNA with random oligonucleotides and for filling-in of 5' protruding ends to blunt-ends, sequencing ssDNA and dsDNA.

Caracteristics

 

  • Storage buffer: 50 mM potassium phosphate (pH 7.0), 0.25 mM DTT, 50% glycerol
  • 10x buffer: 100 mM Tris-HCL (pH 7.5), 70mM MgCl2, 1 mM DTT
  • Unit: One unit is the amount of enzyme to incorporates 10 nmol of total nucleotide into acide insoluble form in 30 min at 37°C
  • QC data: Nuclease activity is not detected.
  • General Use: Use 1 ml of Klenow fragment in a 20 ml of reaction.

 

Application

  • Fill-in 5' overhangs
  • Synthesis probe by random primers labeling
  • Sequencing ssDNA and dsDNA
  • Site-directed mutagenesis

Storage Condition

  • store at -20°C

Klenow Fragment (10 U/ul)
Ordering Information | Cat No 27042| Size 300U/§i
145.00 €
Add to Cart

 
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