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i-genomic Soil DNA Extraction Mini Kit
Ordering Information
Cat No Size
17421 50 col.


Price

265.00 €

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PROTOCOL

Description

The i-genomic Soil DNA Mini Kit isolates genomic DNA and metagenome from various soil samples. A number of types of soil sample can be dealt with the i-genomic Soil DNA Mini Kit, containing common soil samples and high humic acid content such as compost and manure.
The humic acid is one of the major components of humic substance which are dark brown and major constituents of soil organic matter humus that contributs to soil chemical and physical quality and are also precursors of some fossil fuels.
A Humic acid may chelate multivalent cation such as Mg2+, Ca2+ and Fe2+. Therefore this contaminant can inihibit PCR amplification and DNA-protein interacted downstream application.
The i-genomic Soil DNA Mini Kit removes humic acid and PCR inhibitors using chemical and physical procedures during lysis. The isolated DNA is collected on a silica membrane in a spin column procedure. A rapid, inexpensive, convenient DNA extraction method has been developed that is applicable to various soil types. Total genomic DNA can be purified from all the various microorganisms in soil, such as bacteria, fungi and algae. Eluted DNA is high quality and ready for PCR amplification, restriction analysis, electrophoresis, RAPD and any other downapplication.


Characteristics

All types of soil can be used with the i-genomic Soil DNA MiniKit, including the common soil samples and difficult soil samples with high concentration of the humic acid contents such as compost and manure.
The i-genomic Soil DNA Mini Kit removes the humic acid and PCR inhibitors by the chemical and physical process during lysis step.
Rapidly purify the DNA upto one hundred milligram(100mg) of the soil in less than 40 minutes.
The purified DNA is free from the most inhibitors including the humic acid, and can be used directly in the downstream applications including the PCR.

Kit Contents

* Buffer EG

22 ml

* Inhibitor Remover

5 ml

* Buffer EPT

6 ml

* Buffer EB

16ml

* Buffer EWA

12 ml

* Buffer EWB

10 ml

* Buffer EE

20 ml

* Sample Grinder Tube

50 ea

* Spin Columns (Violet color O-ring)

50 columns.

* Collection Tubes (2ml tubes)

100 ea


Results

i-genomic Soil DNA Extraction Mini Kit
Ordering Information | Cat No 17421| Size 50 col.
265.00 €
Add to Cart

 
Instruments & Hardware
Purification Kits
Pipettors Xiril
Extraction Kits