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DNA-maxi SV Plasmid DNA Purification Kit
Ordering Information
Cat No Size
17253 12 col.


Price

181.00 €

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PROTOCOL

Description

DNA-maxi SV Plasmid DNA Purification Kit provides easy and rapid method for the maxi scale preparation of plasmid DNA from bacterial cells. This kit can be used to isolate and purify any plasmid, also can isolated maximum 40 kb size plasmid DNA. The plasmid DNA is free from protein, genomic DNA, and RNA contaminants. This pure plasmid DNA is ready for PCR, cloning, automated or manual sequencing, transfection, synthesis of labeled hybridization probes, electroporation, and enzymatic restriction analysis.

Characteristics

Easy to use - organic extraction or ethanol precipitation is no required.

  • No phenol or chloroform is used.
  • Spend only 30 min (vacuum protocol), 80 ~ 90 min (spin protocol) to extract plasmid DNA
  • Cell lysates remove easily with Pre Column.
    :After mixing with M3 Buffer, the cellular debris and precipitates should be removed completely not to clog Binding Column in subsequent binding. Pre Column facilitates the clearance of the lysate by filtration instead of laborious incubation on ice and centrifugation which has been used widely in traditional methods.
  • Plasmid DNA binds selectively to silica membrane.
  • This column system applies spin and vacuum protocol.

Kit Contents
- Store at RT except for M1 Buffer. M1 Buffer should be stored at 4 after adding RNase A Solution.

M1 Buffer (Resuspension Buffer)
Before use, add 4 of RNase A solution to M1 Buffer. Then, store at 4C.

80 ml
M2 Buffer (Lysis Buffer)
Check M2 Buffer for SDS precipitation due to low storage temperature, in which case it is necessary to dissolve the SDS by warming to 37C.
80 ml
M3 Buffer (Neutralization Buffer) 80 ml
Washing Buffer A
endA+ strains such as HB101, the JM series strains, PR series strains and some other wide-type stains have high endonucleases activity. Endonucleases that can degrade plasmid DNA are essentially removed by Washing Buffer A of DNA-midiTM SV Kit.
300 ml
(150 ml x 2ea )
Washing Buffer B
Before use, if 40ml of Washing Buffer B's bottle, add 160ml of absolute EtOH. And if 20ml of Washing Buffer B's bottle, add 80ml of absolute EtOH.
60 ml
(30 ml x 2ea)

Elution Buffer
DNase / RNase free Ultra-Pure solution.
30 ml
RNase A Solution (10mg/ml)
After receiving, store at 4C.
6 ml
Pre Column (Clear Color)
Inserted into the 50ml disposable tube (collection tube)
12 Col.
Binding Column (Yellow Color)
Inserted into the 50ml disposable tube(collection tube)
12 Col.

Shape and application of Pre & Binding Columns

Fig. 1. Shape and application of Pre & Binding Column
(a) Normal shape of Pre-filtration column & Binding column
Yellow color : Binding Column, Clear color : Pre Column
(b) Shape of 50 tube application (Protocol A)
(c) Shape of vacuum manifold application (Protocol B)

Tendency of plasmid DNA yield & purity from plasmid size

[Table. 1] Yield & purity of various size plasmid DNA isolated from DH5.


Tendency of plasmid DNA from various E.coli strains

[Table. 2] Yield & purity of plasmid DNA isolated from various E. coli host strain


in vitro Translation with purified plasmid DNA

Fig. 2. in vitro translation experiment with GenelatorTMin vitro transcription Translation Kit (iNtRON, Cat. No. 12011 / 12012)
The purified EGFP plasmid DNA from DNA-maxiTM SV Plasmid DNA Purification K and DNA-spinTM Plasmid DNA Purification Kit (iNtRON, Cat. No. 17093) were applied in vitro translation.

(a) UV detection
(b) 12% SDS-PAGE gel running
PC, Positive control (ultra pure pEGF); NC, Negative control (DW); C1 & C2, Control (DNA-spinTM Plasmid DNA Purification Kit); T1, DNA midiTM SV Plasmid DNA Purification Kit (iNtRON, Cat. No. 17252); T2, DNA-maxiTM SV Plasmid DNA Purification Kit

DNA-maxi SV Plasmid DNA Purification Kit
Ordering information | Cat No 17253 | Size 12 col.
181.00 €
Add to Cart

 
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