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Microarray Substrates & Slides - SuperMask™ 4 & 12

SuperMask™ 4 and 12 Substrates are an exciting new innovation from ArrayIt® that provide atomically flat glass substrate slides with ultra-thin hydrophobic masks to create multiple reaction areas or wells on a single substrate slide. SuperMask™ allows users to print and react multiple microarrays per substrate slide, reducing surface chemistry cost for high-throughput analysis for research and diagnostics. The SuperMask™ hydrophobic boundaries are highly durable and available with SuperClean 2, SuperAmine 2, SuperAldehyde 2, SuperEpoxy 2 and SuperStreptavidin surface chemistries for the full spectrum of DNA and protein microarray applications.

Product Description
SuperMask™ Substrates are manufactured using ArrayIt® SuperClean 2 glass substrates that provide superior homogenous topography for DNA and protein microarray applications. SuperMask™ Substrates are designed to empower processing and analysis of multiple microarray experiments on a single microarray substrate slide 25 x 76 mm. Round reaction areas promote even distribution of hybridization mixture across the entire microarray surface area with or without a cover slip and hybridization cassette.

These substrate slides are available in all surface chemistries to meet a wide variety of microarray applications. The substrates are partitioned into individual wells using an ultra-thin 75 µm chemically resistant hydrophobic mask permanently attached to the substrate slide. At 75 µm in height, the hydrophobic mask is sufficiently thin to fit into any microarray printer or scanner, and yet of sufficient height and hydrophobicity to prevent cross contamination with our without cover slips. The matte black mask finish and unique chemical properties prevent contributions to background noise in microarray scans.

Product Use
SuperMask™ 4 Substrates slides have four 12 mm round reaction areas. SuperMask™ 12 Substrate Slides have twelve 6mm round reaction areas. Microarrays are spotted in each reaction area (well) and binding reactions are performed in each well with the SuperMask™ hydrophobic mask serving to partition each wells on the substrate to prevent cross contamination between experiments. Each well can have the same microarray.  Figures 2 and 3 below provide some guidelines on how to manufacture microarrays in each well.  SuperMask™ Substrate Slides may also be used in conjunction with 6 or 12 mm round cover slips using standard protocols and tools such as microarray hybridization cassette chambers, wash stations and microarray centrifuge.  These tools provide for efficient low volume reactions and easy processing.  In addition to the 4 and 12-well patterns, custom SuperMask™ Substrate patterns can easily be manufactured to meet your specific needs. Please contact us directly at
Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. to discuss custom SuperMask™ designs.

 Figure 1. Round reaction areas on these SuperMask™ Substrate Slides facilitates an even distribution of liquid across all spots on the microarray.

Microarrayer Programming and Detection
Manufacturing microarrays into the SuperMask™ Substrates can be accomplished easily with both the SpotBot® 2 and NanoPrint microarrayer product lines. Hybridized microarrays can be scanned using our SpotLight and Laser scanners, or microarray scanners from third parties that support the standard glass substrate slide format. See Figure 1 for the physical layout of SuperMask™ 4 Substrates and refer to Figure 2 for the physical layout of SuperMask™ 12 Substrates. Figure 3 and 4 show source plate loading pin configurations.

 Figure 2. Shown is the physical layout of SuperMask™ 4 Substrates. SuperMask™ 4 contains 4 wells in a 1 x 4 pattern at 13.5mm center-to-center spacing. This makes high throughput microarray manufacturing with up to 4 Micro Spotting Pins set at 13.5 mm center-to-center spacing using a 946 Micro Spotting Device and NanoPrint or other microarrayer (pins pattern shown in red right).  SuperMask™ 4 Substrates contain a corner chamfer in the upper right corner of the substrate, which allows the user to unambiguously identify the side that contains the SuperMask™ hydrophobic partition as the partition always faces side-up when the corner chamfer appears in the upper right corner as the user holds the substrate. The SuperMask™ hydrophobic partition is 75 µm thick on a 0.94 mm glass substrate for a total thickness of approximately 1.015 mm. The 25 x 76 x 1.015 mm SuperMask™ Substrates are compatible with all industry standard open platform hardware and software products including all major brands of microarrayers and scanners.

 Figure 3. Shown is the physical layout of SuperMask™ 12 Substrates. SuperMask™ 12 contains 12 wells in a 2 x 6 pattern at 9mm center-to-center spacing. This makes high throughput microarray manufacturing with up to 12 Micro Spotting Pins set at 9 mm center-to-center spacing using a 946 Micro Spotting Device and NanoPrint or other microarrayer (pins pattern shown in red right). SuperMask™ 12 Substrates contain a corner chamfer in the upper right corner of the substrate, which allows the user to unambiguously identify the side that contains the SuperMask™ hydrophobic partition as the partition always faces side-up when the corner chamfer appears in the upper right corner as the user holds the substrate. The SuperMask™ hydrophobic partition is 75 µm thick on a 0.94 mm glass substrate for a total thickness of approximately 1.015 mm. The 25 x 76 x 1.015 mm SuperMask™ Substrates are compatible with all industry standard open platform hardware and software products including all major brands of microarrayers and scanners.

 Figure 4.  For high throughput microarray manufacturing of the same set of samples on the on the SuperMask 4 Substrate Slide, configure the 384 well source plates to have the same set of samples in each 4 well sample pickup of the pin tool.  MMP384 well source plates are preferred because of their low volume handling properties and compatible pin configuration.

Figure 5.  For high throughput microarray manufacturing of the same set of samples on the on the SuperMask 12 Substrate Slide, configure the 96 or 384 well source plates to have the same set of samples in each 12 well sample pickup of the pin tool.  MMP384 well source plates are preferred because of their low volume handling properties.

SuperMask™ Surface Chemistry Selection and Product Use
Users select the surface chemistry required for a particular application, we are happy to guide end users through surface chemistry selection. Please contact us at
Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. for a quick response on what surface chemistry is best for any particular application. Please follow these links to the various surfaces that we make and their protocols.  SuperClean 2, SuperAmine, SuperAmine 2, SuperAldehyde 2, SuperEpoxy 2 or SuperStreptavidin are available. ArrayIt® Super Microarray substrates support the full gamut of DNA and protein microarray applications, as well as many other applications.  SuperMask substrates are compatible with ArrayIt Brand Hybridization Cassettes, Wash Stations and Microarray Centrifuge for drying.   Please contact us directly if you would like some assistance selecting a custom well pattern or surface chemistry required for your application, either by e-mail Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. or by telephone (408-744-1331).

Ordering Information - SuperMask 4

 

Product

Description

Catalog ID

Cover slips

Individually packaged 12 mm round cover slips, sterile and ready to use, 50 cover slips per package.

CCSL12

SuperClean 2 SuperMask™ 4

SuperMask™ 4 SuperClean 2 microarray substrate with 4 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMC2M4

SuperClean 2 SuperMask™ 4 Barcoded

SuperMask™ 4 SuperClean 2 microarray substrate with 4 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm. barcoded, 25 substrates per box.

SMC2M4BC

SuperAmine
SuperMask™ 4

SuperMask™ 4 SuperAmine microarray substrate with 4 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperAmine surface chemistry, 25 x 76 mm, 25 substrates per box.

SMMM4

SuperAmine
SuperMask™ 4 Barcoded

SuperMask™ 4 SuperAmine microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine surface chemistry, 25 x 76 mm, Barcoded, 25 substrates per box.

SMMM4BC

SuperAmine 2 SuperMask™ 4

SuperMask™ 4 SuperAmine 2 microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMM2M4

SuperAmine 2 SuperMask™ 4 Barcoded

SuperMask™ 4 SuperAmine 2 microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMM2M4BC

SuperAldehyde 2 SuperMask™ 4

SuperMask™ 4 SuperAldehyde 2 microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMA2M4

SuperAldehyde 2 SuperMask™ 4 Barcoded

SuperMask™ 4 SuperAldehyde 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMA2M4BC

SuperEpoxy 2 SuperMask™ 4

SuperMask™ 4 SuperEpoxy 2 microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SME2M4

SuperEpoxy 2 SuperMask™ 4 Barcoded

SuperMask™ 4 SuperEpoxy 2 microarray substrate with 4 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SME2M4BC

SuperStreptavidin
SuperMask™ 4

SuperMask™ 4 Substrate with SuperStreptavidin surface chemistry. 4  hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, 25 substrates per box.

SMSM4

SuperStreptavidin
SuperMask™ 4 Barcoded

SuperMask™ 4 Substrate with SuperStreptavidin surface chemistry. 4  hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMSM4BC

 

Ordering Information - SuperMask 12

Product

Description

Catalog ID

Cover slips

Individually packaged 6 mm round cover slips, sterile and ready to use, 50 cover slips per package.

CCSL6

SuperClean 2
SuperMask 12

SuperMask 12 SuperClean 2 microarray substrate with 12 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMC2M12

SuperClean 2
SuperMask 12
Barcoded

SuperMask 12 SuperClean 2 microarray substrate with 12 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm. barcoded, 25 substrates per box.

SMC2M12BC

SuperAmine
SuperMask 12

SuperMask 12 SuperAmine microarray substrate with 12 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperAmine surface chemistry, 25 x 76 mm, 25 substrates per box.

SMMM12

SuperAmine
SuperMask 12
Barcoded

SuperMask 12 SuperAmine microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine surface chemistry, 25 x 76 mm, Barcoded, 25 substrates per box.

SMMM12BC

SuperAmine 2
SuperMask 12

SuperMask 12 SuperAmine 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMM2M12

SuperAmine 2
SuperMask 12
Barcoded

SuperMask 12 SuperAmine 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMM2M12BC

SuperAldehyde 2
SuperMask 12

SuperMask 12 SuperAldehyde 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMA2M12

SuperAldehyde 2
SuperMask 12
 Barcoded

SuperMask 12 SuperAldehyde 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMA2M12BC

SuperEpoxy 2
SuperMask 12

SuperMask 12 SuperEpoxy 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SME2M12

SuperEpoxy 2
SuperMask 12
Barcoded

SuperMask 12 SuperEpoxy 2 microarray substrate with 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SME2M12BC

SuperStreptavidin
SuperMask 12

SuperMask 12 Substrate with SuperStreptavidin surface chemistry. 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, 25 substrates per box.

SMSM12

SuperStreptavidin SuperMask 12
 Barcoded

SuperMask 12 Substrate with SuperStreptavidin surface chemistry. 12 hydrophobic wells in a 2 x 6 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, barcoded, 25 substrates per box.

SMSM12BC

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund.  Any misuse of this product including the use of the wrong protocols is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.