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Microarray Substrates & Slides - SuperMask™ 16 & 24

SuperMask™ Substrates are an exciting new innovation from ArrayIt® that provide atomically flat glass substrate slides with ultra-thin hydrophobic masks for 16 or 24 or separate reaction areas or wells on a single substrate slide. SuperMask™ allows users to print and hybridize 16 or 24 separate microarrays per substrate slide, allowing high-throughput analysis for research and diagnostics. The SuperMask™ hydrophobic boundaries are highly durable and availaible with SuperClean 2, SuperAmine 2, SuperAldehyde 2 and SuperEpoxy 2 surface chemistries for the full spectrum of DNA and protein microarray applications.

Product Description
SuperMask™ Substrates are manufactured using ArrayIt® SuperClean 2 glass substrates that provide superior homogenous topography for DNA and protein microarray applications. SuperMask™ Substrates are designed to empower processing and analysis of multiple samples of 16 or 24 individual microarray experiments on a single microarray substrate slide 25 x 76 mm. Available in all surface chemistries to meet every application, the substrates are partitioned into individual wells using an ultra-thin 75 µm chemically resistant hydrophobic mask.  At 75 µm in height, the hydrophobic mask is sufficiently thin to fit into any microarray printer or scanner, and yet of sufficient height and hydrophobicity to prevent cross contamination. The matte black mask finish and unique chemical properties prevent contributions to background noise in microarray scans.

Product Use
SuperMask™ Substrate Slides may be used either with or without additional hardware to partition the wells. In the absence of additional hardware, the SuperMask™ well volume is 10-100 µl depending on the surface chemistry and reaction buffer used, and such reactions are performed using a small droplet in each well with the SuperMask™ hydrophobic mask serving to partition each of the 16 or 24 wells on the substrate. SuperMask™ Substrate Slides may also be used in conjunction with the multiple microarray hardware products MMH4x24, MMH4x16, AHC1x24 and AHC1x16. In addition to the 16-well and 24-well patterns, custom SuperMask™ Substrate patterns can be manufactured to meet your specific needs. Please contact us directly at
Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. to discuss custom SuperMask™ designs.

Microarrayer programming and detection
Manufacturing microarrays into the 16-well and 24-well SuperMask™ Substrates can be accomplished easily with both the SpotBot® 2 and NanoPrint microarrayer product lines. Hybridized microarrays can be scanned using our SpotLight and Laser scanners, or microarray scanners from third parties that support the standard glass substrate slide format. See Figure 1 for the physical layout of SuperMask™ 16 and SuperMask™ 24 Substrates.


Figure 1. Shown is the physical layout of SuperMask™ 16 (right) and SuperMask™ 24 (left) Substrates. SuperMask™ 16 contains 16 wells in a 2 x 8 pattern, with the 2 x 8 reactive area spanning 16 x 70 mm and each well measuring 7 x 7 mm. SuperMask™ 24 contains 24 wells in a 3 x 8 pattern, with the 3 x 8 reactive area spanning 22 x 70 mm and each well measuring 6 x 7 mm. SuperMask™ Substrates contain a corner chamfer in the upper right corner of the substrate, which allows the user to unambiguously identify the side that contains the SuperMask™ hydrophobic partition as the partition always faces side-up when the corner chamfer appears in the upper right corner as the user holds the substrate. The SuperMask™ hydrophobic partition is 75 µm thick on a 0.94 mm glass substrate for a total thickness of approximately 1.015 mm. The 25 x 76 x 1.015 mm SuperMask™ Substrates are compatible with all industry standard open platform hardware and software products including all major brands of microarrayers and scanners.

How to select the right SuperMask™ product
Users need to select the number of wells (16 or 24) and the surface chemistry required for a particular application (SuperClean 2, SuperAmine, SuperAmine 2, SuperAldehyde 2 or SuperEpoxy 2). ArrayIt® Super Microarray substrates support the full gamut of DNA and protein microarray applications, as well as many other applications. Please contact us directly if you need assistance selecting the well pattern or surface chemistry required for your application
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Figure 2. Shown are the surface chemistries available for the SuperMask™ 16 and SuperMask™ 24 Substrates, including polished atomically flat glass ready for custom chemistry (SuperClean), polished atomically flat glass containing positively charged reactive amine groups for electrostatic binding of DNA, proteins, and other molecules (SuperAmine), polished atomically flat glass containing reactive aldehyde groups for covalent binding of DNA, proteins, and other molecules (SuperAldehyde), and polished atomically flat glass containing highly reactive epoxide groups for covalent binding of DNA, proteins, and other molecules (SuperEpoxy). ,SuperMask™ 16 contains 16 wells in a 2 x 8 pattern and SuperMask™ 24 contains 24 wells in a 3 x 8 pattern. The SuperMask™ hydrophobic partition is 75 µm thick on a 0.94 mm glass substrate for a total thickness of approximately 1.015 mm. The 25 x 76 x 1.015 mm SuperMask™ Substrates are compatible with all industry standard open platform hardware and software products including all major brands of microarrayers and scanners. Please see the Ordering Information table to select the SuperMask™ product that fits your needs.

Recommended Equipment and Reagents
NanoPrint™ Microarrayers
SpotBot® 2 Personal Microarrayers
SpotLight™ Microarray Scanners
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuge
Protein Printing Buffer
BlockIt™ Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
BlockIt Blocking Buffer
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers

Ordeing Information

Product

Description

Catalog ID

SuperClean 2
SuperMask™ 16

SuperMask™ 16 SuperClean 2 microarrray substrate with 16 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMC2M16

SuperAmine
SuperMask™ 16

SuperMask™ 16 SuperAmine microarrray substrate with 16 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperAmine surface chemistry, 25 x 76 mm, 25 substrates per box.

SMMM16

SuperAmine 2
SuperMask™ 16

SuperMask™ 16 SuperAmine 2 microarrray substrate with 16 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMM2M16

SuperAldehyde 2
SuperMask™ 16

SuperMask™ 16 SuperAldehyde 2 microarrray substrate with 16 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMA2M16

SuperEpoxy 2
SuperMask™ 16

SuperMask™ 16 SuperEpoxy 2 microarrray substrate with 16 hydrophobic wells in a 2 x 8 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SME2M16

SuperStreptavidin
SuperMask™ 16

SuperMask™ 16 SuperStreptavidin microarrray substrates with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, 25 substrates per box.

SMSM16

SuperClean 2
SuperMask™ 24

SuperMask™ 24 SuperClean 2 microarrray substrate with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperClean 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMC2M24

SuperAmine
SuperMask™ 24

SuperMask™ 24 SuperAmine microarrray substrate with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMMM24

SuperAmine 2
SuperMask™ 24

SuperMask™ 24 SuperAmine 2 microarrray substrate with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperAmine 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMM2M24

SuperAldehyde 2
SuperMask™ 24

SuperMask™ 24 SuperAldehyde 2 microarrray substrate with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperAldehyde 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SMA2M24

SuperEpoxy 2
SuperMask™ 24

SuperMask™ 24 SuperEpoxy 2 microarrray substrate with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperEpoxy 2 surface chemistry, 25 x 76 mm, 25 substrates per box.

SME2M24

SuperStreptavidin
SuperMask™ 24

SuperMask™ 24 SuperStreptavidin microarrray substrates with 24 hydrophobic wells in a 3 x 8 pattern on an atomically flat glass substrate with SuperStreptavidin surface chemistry, 25 x 76 mm, 25 substrates per box.

SMSM24

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund.  Any misuse of this product including the use of the wrong protocols is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.