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Plasmid DNA Purification Kits


Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Short Protocol
  • Equipment and Reagents
  • Troubleshooting Tips
  • Ordering Information
  • Storage Conditions
  • Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains a complete set of protocols including the steps and principles needed to use Arrayit’s ArrayIt® brand Plasmid DNA Mini-Prep.

Quality Control
Arrayit assures the performance of these products.  The finest scientific research went into the development of these products. Rigorous quality control monitoring on a lot-by-lot basis guarantees that the columns, plates, buffers, and protocols conform to the highest industry standards.


Figure 1. Showing the high reliability of the kit, the error is < 5%.
 

Product Description

20% higher yield than other kits
Quicker protocol
High quality of preps (all downstream applications)
Processing of up to 6 ml culture
For E. coli and other Gram-negative bacteria

Figure 2.  Comparison of the ArrayIt kit to the leading competitor.
 

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail: Cette adresse email est protĂ©gĂ©e contre les robots des spammeurs, vous devez activer Javascript pour la voir. (under the subject heading please type ArrayIt technical assistance).  By telephone: (408) 744-1331, Monday—Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

Short Protocol

Centrifuge culture at >13,000 rpm for 30-60 s.
Resuspend pellet in 250 µl buffer 1.
Mix with 250 µl buffer 2.
Mix with 350 µl buffer 3.
Centrifuge at >13,000 rpm for 7 min.
Decant supernatant in mini Spin column and centrifuge at >13,000 rpm for 30 s.
Wash column with 700 µl buffer 4 (30 s, >13,000 rpm).
Dry membrane at >13,000 rpm for 1 min.
Pipette 30-100 µl buffer 5 or water onto membrane, incubate for 1 min and centrifuge at >13,000 rpm for 1 min.
         Time: 15 min for 2 x 2 ml culture; 45 min for 20 parallel preparations


Figure 3. Showing the high quality of plasmid DNA.
 


Figure 4. Plasmid Mini Prep works great for both low copy and high molecular size plasmids.
 


Figure 5. Useful for all E. coli cloning strains.
 


Figure 6. Data from low copy number plasmids from E. coli and other bacteria.
 

Equipment and Reagents

Troubleshooting Tips

Ordering Information

Product

Description

Catalog ID

Price per kit (US dollars)*

Plasmid DNA Purification Kit

50 reactions in a single tube kit, reagents, buffers, columns and handbook.

PDP50

$110

Plasmid DNA Purification Kit

250 reactions in a single tube kit, reagents, buffers, columns and handbook.

PDP250

$410

Discounts:
10-49 kits, 10% discount
50-99 kits, 15% discount
100+ kits, 20% discount

Storage Conditions
Kits should be stored dry at room temperature (20-25°C). The kits perform well across a wide range of ambient temperatures and relative humidity.  The kits are nuclease-free, sterile, and have a shelf life of one-year from the date of purchase.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with our ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund.  Any misuse of this product including deviations from our protocols is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

 
Instruments & Hardware
Purification Kits
Pipettors Xiril
Useful Tools & Equipment
Extraction Kits