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Services - Protein Microarrays

Arrayit offers custom protein microarray manufacturing. Our core microarray manufacturing  technologies are compatible with all modern microarray detection methods including fluorescence, chemiluminescence, colorimetric, RLS, SPR and mass spectoscopy. The type of assay and detection method will determine what surface chemistry is used to manufacture the microarrays.

Our universal microarray platform empowers us to provide:

  • Multiplexed Microarray Based ELISA Assays (microspot ELISA)
  • Antibody Microarrays
  • Antigen Microarrays
  • Whole Proteome Microarrays
  • Reverse Phase Microarrays
  • Peptide microarrays
  • and others…

Protein Microarray Applications include but are not limited to:

  • Expression Profiling
  • Serum Based Diagnostics
  • Protein-protein binding assays
  • Drug-target binding
  • Receptor epitope binding

Pharmaceutical and biotechnology researchers use protein microarrays to streamline drug target identification, selection, validation, and predictive testing.

Figure 1: Libraries of express proteins are easily printed into microarrays with the NanoPrint Microarrayer.  For detection of the printed proteins, samples can be directly labeled or a non-label detection technique can be used. A variety of protein- protein, protein-drug, and protein-small molecule interactions can be implemented.   These microarrays can be used to analyze antibody specificity.

Figure 2: Our technologies are suitable miniaturizing and multiplexing assays that use both antigens and antibodies.  We can implement MicroSpot ELISA (micro enzyme linked immunosorbent assay) and antibody microarrays. Detection can be fluorescent or colorimetric using secondary antibodies labeled with AP, HRP, biotinylated secondary antibodies that bind labeled streptavidin or direct fluorescent labeling.  A variety of sandwich assays can be implemented in multiplexed fashion. As many as 24 identical microarrays can be run per slide.

Figure 3: Our microarray technologies can be used to implement reverse phase microarrays. These microarrays are used to profile bound complex cell lysates, cells captured by LCM or serum samples printed into microarrays. The bound samples can be probed with antibodies for the detection of antigens present in the printed samples. Detection can be accomplished a variety of ways including use of a fluorescently labeled secondary antibody.
Figure 4: Synthetic proteins, peptides and engineered proteins to detect the presence of proteins in complex samples can also be implemented. Unique protein binding events, epitope mapping studies, small molecule binding events or protein/protein interactions can be detected.

We have several tools available for running microarrays in parallel.  Pictured below is our tool ACH4x24, it has the same footprint of a standard 96 well plate, yet holds up to 24 microarrays on 4 microarray slides. Compatible with most all standard 96 well plate ELISA based processing automation. contact us at Cette adresse email est protégée contre les robots des spammeurs, vous devez activer Javascript pour la voir.

Running Microarrays in parallel

Microarray Manufacturing Consulting Services - Get direct and practical “how to” information from the worlds leader of tools, kits and reagents used in microarray manufacturing.

Figure 2. 100 micron diameter spots of Ten Fold Dilution series of Human IgG starting at 1 µg/µl Printed in Protein Printing Buffer Plus left to right top down.  Microarrays manufactured using a NanoPrint Microarrayer, Professional Printhead and 946MP3 Microarray Printing Pins. SuperEpoxy2 Microarray Substrates, Blocked wtih BlockIt Plus, reacted with Anti-Human IgG Conjugated to Cy3 using diluted 1 to 2000 in Protein Microarray Reaction Buffer using an RC1x24 Microarray Reaction Cassette. Microarray Scan at 9 um resolution using an ArrayWell Microarray Scanner.

Microarray pads

Protein Microarray Immunoassay Experimental Design

Arrayit's microarray platform is highly flexible and powerful suite of technologies for miniaturizing and multiplexing existing assays such as those for gene expression, genotyping, immunoassays and others.  Microarray immunoassay test development requires a little work up front, but it's fun to do. Here is a list of questions to help guide the process of experimental design.  You don't have to answer all these questions to have a legitimate project, questions are not applicable to all projects.  We sign confidentiality agreements, we don't expect customers to disclose things they do not wish to disclose! Contact: Cette adresse email est protégée contre les robots des spammeurs, vous devez activer Javascript pour la voir. or phone 408-744-1331.

  • How will the microarray assay be used and what are the goals of the experiments or tests?
  • What is the specific biomolecule or analyte that you want to measure?
  • Have ELISA assays been run with the sample types?
  • What capture analytes are we printing into a microarray?
    • What are the characteristics of the capture analytes for the microarray?
    • Antigen, antibody, cell lysate, peptide...other?
    • If antibodies, do you have a secondary antibody for each capture antibody?
    • Amount of purified capture analyte available?
    • If the analytes are peptides do you have all the amino acid sequences?
    • Are there any general toxicity or safety issues associated with the handling or use of the analyte?
    • Storage buffer (including pH)?
    • Soluble in water or is another buffer required?
    • Storage conditions?
  • If analyte is lyophilized, where any stabilizers used?
  • What is the origin of the sample to be tested...blood, serum, plasma, water, tissue culture supernatants, etc.)?
  • Do the samples to be tested on the microarray have to be pre-treated, such as extractions or centrifugations? If yes, what are they?
  • What is the expected outcome of the immunoassay you want to develop?
  • Are we developing an assay that will require routine testing?
    • How many samples will be tested overall?
    • How many samples do you want to test for at one time?
    • Do you require automation, or is manual processing OK?
    • Did you want to outsource the testing or do parts or all of it?
  • Do you have a microarray scanner or other instrument for reading the test?
  • Are you planning fluorescent, colorimetric, chemiluminescent, SPR or other detection platform?
  • Does your test require FDA approval (or will it in the future)?
  • Is there anything else important you think we should know?